NucliSens EasyQ方法在HIV-1病毒载量检测中的应用及质量控制  

Application and quality control of NucliSens EasyQ method in the HIV-1 viral load detection

作　　者：张佳峰[1] 郭志宏[1] 黄晶晶[1] 丁晓贝[1] 郑锦雷[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310051

出　　处：《中国卫生检验杂志》2012年第11期2682-2684,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:利用NucliSens EasyQ方法进行HIV-1病毒载量检测,探索病毒载量的指导意义,并分析实验质控的关键点。方法:对2008年3月-2010年12月浙江省各地送检的2129例血浆样本进行HIV-1病毒载量检测,对样本信息和实验结果进行统计分析。结果:研究对象的CD4淋巴细胞数(X)与病毒载量(Y)呈负相关,线性方程为lg(Y)=-0.002X+4.397(X<800),R=0.342,P<0.01,N=431。实验结果的总无效率为5.0%(106/2129),无效结果主要是磁性硅胶或硫氰酸胍残留引起(占63.2%),代码4(无扩增)和代码9(弱扩增)最常见共占83.0%(88/106)。结论:病毒载量对病程监控和预测及抗病毒治疗具有重大的指导意义;EasyQ方法实验质控关键点是避免磁性硅胶和硫氰酸胍的残留。Objective：To investigate the guiding significance of HIV viral load（VL） level by the NucliSens EasyQ method,additionally,to analyze the key points in the quality control of the experiment.Methods： The plasma samples submitted in Zhejiang Province from March 2008 to December 2010 were detected for HIV-1 viral load,and experimental results were analyzed statistically combined with sample information.Results： There was significantly negative correlation between the CD4 lymphocytes count X and viral load Y（lg（Y）=-0.002X＋4.397,X800,R=0.342,P0.01,N=431）.The total rate of invalid results was 5.0%（106/2129）,most were caused by the residual magnetic silica or guanidine thiocyanate（accounting for 63.2%）.Code 4（no amplification） and code 9（weak amplification） were the most common for invalid results,accounting for 83.0%（88/106）.Conclusion： Viral load was of great significance in monitoring and prediction of disease and antiretroviral treatment for HIV-infected patients.The key point in the quality control of experiment was to avoid the residual magnetic silica and guanidine thiocyanate.

关 键 词：EasyQ HIV 病毒载量 无效结果 

分 类 号：R512.91[医药卫生—内科学]