密林熊蜂雄性成蜂生殖系统发育动态  

Development of the reproductive system in the male adult of bumblebee Bombus patagiatus (Hymenoptera:Apidae)

作　　者：郭媛[1] 刘耀明[2] 邵有全[1] 

机构地区：[1]山西省农业科学院园艺研究所,太原030031 [2]山西大学应用生物学研究所,太原030006

出　　处：《昆虫学报》2012年第11期1322-1330,共9页Acta Entomologica Sinica

基　　金：公益性行业(农业)科研专项(201203080);现代农业产业技术体系(蜜蜂)建设项目(CARS-45-KXJ5)

摘　　要：为了研究密林熊蜂Bombus patagiatus Nylander雄蜂出房后生殖系统发育变化过程,本研究首次对密林熊蜂雄性成蜂(1-15日龄)生殖系统的精液量、精子数、性腺大小、储精囊大小等进行了测定。结果显示:雄性成蜂在1日龄时精液量最大,达到5.95±0.39μL;镜检精子数在2日龄最多;而精液中活动精子数出现波浪式的循环趋势,9日龄达到最大值,为25.93±1.06个;精液中精子总数在9日龄达到最大值,为160.67±17.11万个;活动精子百分比以8、9日龄最高,分别达到86.38%±2.02%及86.45%±2.50%;性腺长度在1日龄时最小,为1522.01±37.93μm;储精囊的中部宽度在7日龄最宽,达86.38±2.96μm,但与9日龄相比无显著差异。由此可见,8-9日龄雄性成蜂个体各指标较为稳定,该日龄即为雄性成蜂性成熟且可以交配的时期。本研究结果为密林熊蜂人工繁育提供了理论依据。To study the developmental process of the reproductive system in the male adult of bumblebee Bombus patagiatus Nylander following emergence, semen volume, sperm count, gonad and spermatheca size of the reproductive system in virgin male adults at different day-old （ 1 - 15 day-old） were measured. The results showed that the volume of seminal fluid of drones at 1 day-old （ 5.95 ± 0.39 μL） was maximal, while the sperm count of drones at 2 day-old reaches the peak under microscope. The number of motile sperms in the semen presented repeated wave trends. The motile sperm count （25.93 ± 1.06） and the total number of sperm in the semen （ 160.67 ± 17.11 ten thousand） reached the maximum at 9 day-old. The highest percentages of motile sperms were 86. 38% ± 2.02% and 86.45% μ 2.50% , respectively, at 8 and 9 day-old. The length of gonad （1 522.01±37.93 μm） was minimum at 1 day- old. The middle width of spermatheca （86.38 ±2.96 μm） reached the maximum at 7 day-old, but had no significant difference with that at 9 day-old. The results indicate that the indexes of the reproductive system are relatively stable in drones at 8 -9 day-old, and drones reach sexual maturity and could mate during this stage. The study provides a theoretical basis for artificial breeding of B. patagiatus.

关 键 词：密林熊蜂 雄性生殖系统 性腺 精子 发育 

分 类 号：Q964[生物学—昆虫学]