黄连解毒汤对感染大肠埃希菌大鼠脑组织TLR9表达的影响  

Effects of HuangLianJieDu Decoction on the Expression of TLR9 in Brain Tissue of Rats Infected with Escherichia coli

作　　者：刘晓强[1] 周宏超[1] 俞亚玲[1] 赵守中[1] 杨鸣琦[1] 李引乾[1] 

机构地区：[1]西北农林科技大学动物医学院,杨凌712100

出　　处：《畜牧兽医学报》2012年第12期1963-1968,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：陕西省科技攻关项目(2009K02-01)

摘　　要：为探讨Toll样受体9(TLR9)及相关炎症因子在脑损伤中的调节机制,采用免疫组织化学SP法对正常大鼠、感染大肠埃希菌大鼠和黄连解毒汤预防大鼠脑组织中的TLR9表达及相关因子的动态变化进行了研究。66只SD大鼠随机分为正常对照组(n=6,腹腔注射生理盐水0.5mL.只-1)、试验组[n=30,腹腔注射大肠埃希菌菌液0.5mL.只-1(2.4×109 CFU.mL-1)]、黄连解毒汤预防组[n=30,每只按12.6mL.kg-1剂量提前6d灌服黄连解毒汤,处理当天再分别腹腔注射大肠埃希菌菌液0.5mL.只-1(2.4×109 CFU.mL-1)]。除对照组外,试验组和预防组又分6个小组,每小组5只大鼠。分别于处理后的3、6、9、12、18、24h随机处死1小组试验组和预防组大鼠及1只对照组大鼠,免疫组化观察脑组织TLR9及相关因子的表达)。结果发现,TLR9在大鼠正常生理状态下有少量表达,感染大肠埃希菌后,TLR9的表达出现明显变化,且这种变化随着时间的推移而越发明显。在感染后3h时TLR9表达量较正常生理状态下显著增加(P<0.05),在6h时开始显著增加,12h时达到高峰,与对照组和试验组相比,均有极显著差异(P<0.01)。预防组与对照组比较无统计学意义(P>0.05)。该研究结果显示黄连解毒汤可通过抑制TLR9的表达对细菌所致的脑损伤产生保护作用。The aim of this study was to investigate the mediation mechanism of toll-like receptor 9 （TLR9） and the related inflammatory factors in brain injury. Immunohistochemical ultra sensi- tive SP method was used to examine the dynamic expression of TLR9 and the related factors in the normal rats, injected with E. coli and HuangLianJieDu Decoction preventive rats in the pres- ent study. 66 SD rats were randomly divided into three groups. The control group （n= 6） was treated with normal saline by intraperitoneal injection. Experimental group （n= 30） was treated with E. coli culture by intraperitoneal injection 0.5 mL per rat （2.4 ×10^9CFU·mL-1）. Huan- gLianJieDu Decoction preventive groups （n =30） was treated with 12. 6 mL · kg-1 orally in advance 6 days, and then were intraperitoneally injected with 0.5 mL （2.4 ×10^9CFU·mL-1） per rat E. coli culture. Each group was divided into 6 sub-groups and each of which has 5 rats. The rats of each experimental group, prevention group and one rat in control group were killed randomly at 3, 6, 9, 12, 18, 24 hours after treated with E. coll. And then the expression of

关 键 词：Toll受体9 黄连解毒汤 脑组织 大肠埃希菌 

分 类 号：S852.21[农业科学—基础兽医学] S853.9[农业科学—兽医学]