射干地龙颗粒的安全药理学分析  

The Safety Pharmacology Analysis of Shegan Dilong Particles

作　　者：王贵波[1] 罗永江 罗超应 李锦宇 郑继方[1] 谢家声[1] 辛蕊华[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,兰州730050 [2]农业部兽用药物创制重点实验室,兰州730050 [3]甘肃省中兽药工程技术研究中心,兰州730050

出　　处：《畜牧兽医学报》2012年第12期1969-1977,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："十二五"国家支撑计划(2011BAD34B03);甘肃省科技支撑项目(1104NKCA094)

摘　　要：为了进一步探讨射干地龙颗粒临床用药的安全性,首次通过无创方式对射干地龙颗粒开展了安全药理学研究。分别按体质量应用高剂量(2.0g.kg-1)、中剂量(1.0g.kg-1)、低剂量(0.5g.kg-1,相当于推荐剂量)的射干地龙颗粒和生理盐水对犬进行灌胃,并观察麻醉犬的平均动脉压、心率、体温、心电标准Ⅱ导联、呼吸频率、潮气量、血氧饱和度、呼吸曲线、尿液11项指标和尿液增质量等相关指标;同时对昆明系小鼠按照上述药物剂量进行灌胃,并观察小鼠的自主活动;通过以上指标的观测来说明药物对心血管系统、呼吸系统、泌尿系统和中枢神经系统的影响。结果表明,按照2.0g.kg-1将射干地龙颗粒进行灌胃,对麻醉犬的心血管系统、呼吸系统和泌尿系统无显著影响;小鼠自主活动结果表明,小鼠活动的总路程和时间及各边、角的路程和时间组间差异不显著(P>0.05),且小鼠的一般行为活动、姿势、步态均表现正常,无肌颤、不安、奔跑、嘶叫、蜷缩竖毛等异常,说明在试验剂量范围内药物对小鼠的中枢神经系统不表现显著影响。结果表明,至少在0.5～2.0g.kg-1范围内给药,射干地龙颗粒对动物心血管系统、呼吸系统、泌尿系统及中枢神经系统均无明显影响,表明其不良反应小,是一种适合于临床应用的、较为安全的药物。In order to study clinical safety of Shegan Dilong particles, the safety pharmacology study was carried out for the first time by non-invasive way. Three doses of Shegan Dilong parti- cles including high dose （2.0 g·kg^-1）, medium dose （1.0g·kg^-1） and low dose （0.5 g·kg^-1 , which is equivalent to the recommended dose） were given to the anesthetized dogs, and there was also a group of anesthetized dogs given saline. Then the mean arterial pressure, heart rate, body temperature, standard Ⅱ lead ECG, respiratory rate, tidal volume, oxygen saturation, respira- tion curve, urine of eleven indicators, urine weight gain and other related indicators of the anes- thetized dogs were observed. Meanwhile Kunming strain mice were given the particles in accord- ance with the above different doses, and the spontaneous activities of mice were observed. The above indicators observed were used to illustrate the impact of the particles on the cardiovascular system, respiratory system, urinary system and central nervous svstem. The results shnwed thatShegan Dilong particles given by oral according to the dose of 2.0 g · kg-1 had no significant effect on the cardiovascular system, respiratory system and urinary system of anesthetized dogs. The results of the mices spontaneous activities also showed that the total distance, the side of distance, the angle of distance and time among the groups had no significant differences （P 〈0.05）. And the general conduct of activities, posture and gait of the mice performed normal, while muscle trembling, anxiety, running, screams and other abnormalities in mice didn＇t exit. It indicated that in the range of the test doses, the particles had no effect on the central nervous system in mice. The results suggested that at Dilong particles had no significant effect on least in the range of 0.5-2.0 g ~ kg-1 the Shegan animal cardiovascular system, respiratory system, urinary system and central nervous system, and it also suggested that the adverse reactions was small. So it�

关 键 词：射干地龙颗粒 安全药理 犬 小鼠 

分 类 号：S853.7[农业科学—临床兽医学]