出生后发育时期济宁青山羊乳腺中ERα和PR的分布及其mRNA表达  

Localization of ERα and PR and Differential Expression of ERα mRNA and PR mRNA in Mammary Gland of Jining Gray Goat during Postnatal Development

作　　者：尹玉涛[1] 王树迎[1] 刘宵[1] 张德敏[1] 侯衍猛[1] 黄丽波[1] 

机构地区：[1]山东农业大学动物科技学院动物生物技术与疾病防治重点实验室,泰安271018

出　　处：《畜牧兽医学报》2012年第12期1984-1990,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30871832);山东省自然科学基金(ZR2009DM027);山东省科技攻关项目(2008GG30008018)

摘　　要：本研究旨在探讨济宁青山羊出生后发育过程中乳腺组织内雌激素受体α(ERα)和孕激素受体(PR)免疫阳性细胞的分布及平均光密度和mRNA分子水平的表达规律。应用SP免疫组化法和图像分析方法检测ERα和PR在济宁青山羊乳腺内的分布;以GAPDH为内参基因,qRT-PCR方法检测ERα和PR mRNA的相对表达量。结果表明,ERα和PR阳性染色主要见于乳腺组织内的血管内皮细胞、基质细胞和腺管上皮细胞的细胞核中,偶见胞浆阳性着色,180日龄在腺泡上皮细胞亦可见PR阳性表达;ERα和PR平均光密度自出生～性成熟期呈现逐渐上升的趋势,且性成熟期显著高于出生当天和初情期(P<0.05),但与180日龄相比无显著差异(P>0.05)。qRT-PCR结果显示,ERαmRNA的表达量自出生～性成熟期呈现逐渐上升趋势,性成熟期最高,极显著高于出生当天和初情期(P<0.01),180日龄略有下降。PR mRNA表达量自出生当天～性成熟期较低且组间差异不显著(P>0.05),180日龄的表达量最高且极显著高于其他的3组(P<0.01)。济宁青山羊出生后发育过程中,ERα对腺管伸长和上皮分化以及PR对腺管分支和腺泡发育起促进作用。The objective of the experiment was to study the localition and the average integrated optical density of ERα and PR immunopositive cells and the abundance of ERα mRNA and PR mRNA in mammary gland during postnatal development in Jining Gray goat. The SP immunohis- tochemical staining and Image analysis methods were used to localize ERa and PR immunoprotec- tive（-ip） cells in mammary gland of Jining Gray goat. The relative expression of ERa mRNA and PR mRNA was analyzed by using the real time fouorescence quantitative PCR with GAPDH as an internal gene. ERα and PR had similar variations, immunoprotective staining was mainly local- ized in the nucleus of blood vessels endothelial cells, stromal cells and ductal epithelial cells of mammary gland, occasionally in cytoplasmic, PR-ip substance also found in alveolar epithelial cells in 180 days. The average optical density was gradually increased from birth day to sexual ma- turity, and it was significantly higher in sexual maturity than that in birth day and puberty （P〈 0.01） ,it was not different with that in 180 days （P〈0.05）. The results of qRT-PCR showed that the abundance of ERα mRNA was gradually increased from birth day to sexual maturity ,and it was significantly higher in sexual maturity than that in birth day and puberty （P〈0.01）, there was a slightly decrease from sexual maturity to 180 days . While the abundance of PR mR- NA from birth day to sexual maturity was lower and there was no significant difference during these groups. In 180 days, it was significantly higher than the other three groups （P〈0.01）. These results indicated that the estrogen receptors of alph and the progesterone receptors could promote the growth of glandular ,the hyperplasia of epithelial cells and the development of lobu- lo-alveolar during postnatal development in Jining Gray goat.

关 键 词：济宁青山羊 乳腺 雌激素受体Α 孕激素受体 免疫组织化学 qRT—PCR 

分 类 号：S827[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]