前列腺素E1联合血必净对肺间质纤维化大鼠转化生长因子β-1的影响  

Effect of prostaglandin E1 combined with Xuebijing injection on transforming growth factor-β1 in rats with pulmonary interstitial fibrosis

作　　者：张万祥[1] 李志军[1] 

机构地区：[1]天津第一中心医院中西医结合科,300192

出　　处：《中华劳动卫生职业病杂志》2012年第12期913-915,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的探讨前列腺素E1联合血必净对急性肺间质纤维化大鼠转化生长因子（TGF-β1 ）和肿瘤坏死因子（TNF-α）表达的影响。方法采用一次性气管内注射博来霉素（1ml／kg）的方法复制大鼠肺间质纤维化模型。将Wistar大鼠108只大鼠随机分为正常对照组、模型组、甲泼尼龙治疗组、血必净治疗组、前列腺素E1治疗组、联合用药组（前列腺素E1联合血必净），每组18只。除正常对照组外，对各组造模大鼠分别予不同处理后第7、14、28天处死。免疫组化染色检测肺组织TGF-β1表达，EusA方法测定模型大鼠支气管肺泡灌洗液（BALF）中TNF-α浓度。结果联合用药组大鼠肺组织各时点TGF-β1表达的巨噬细胞个数均明显低于模型组、血必净组、甲泼尼龙组、前列腺素E1组，差异均有统计学意义（P〈0．05）。第7天时联合用药组BALF中TNF-α浓度明显低于模型组、甲泼尼龙组和前列腺索E1组，差异有统计学意义（P〈0．05）；第14天时，联合用药组BALF中TNF-α浓度明显低于模型对照组，差异有统计学意义（P〈0．05）；第28天时，前列腺素E1组和联合用药组TNF-α水平低于模型对照组，差异有统计学意义（P〈0．05）。结论前列腺素E1联合血必净可明显抑制急性肺间质纤维化模型大鼠肺组织TGF-β1表达，减轻肺泡炎性反应。Objective To investigate the effect of prostaglandins E1 combined with Xuebijing injection on the expression of transforming growth factor-β1 （TGF-β1） and tumor necrosis factor-α （TNF-α） in rats with acute pulmonary interstitial fibrosis. Methods A rat model of pulmonary interstitial fibrosis was established by intratracheal injection of bleomycin （ 1 ml/kg）. One hundred and eight Wistar rats were randomly divided into six groups with 18 in each group, which were normal control group, model group, hormone （methylprednisolone） treatment group, Xuebijing treatment group, prostaglandin E1 treatment group and combination treatment group （prostaglandin E1 and Xuebijing injection）. Except for those in the normal control group, the rats in each group were sacrificed on the 7th, 14th and 28th day after treatment. The TGF-β1 expression in lung tissue was measured by immunohistochemical staining. The TNF-α concentration in bronchoatveolar lavage fluid （BALF） of rat model was determined by enzyme-linked immunosorbent assay. Results The combination treatment group showed significantly more macrophages with TGF-β1 expression in lung tissue at each time point, as compared with the model group, Xuebijing treatment group, methylprednisolone treatment group and prostaglandin E1 treatment group （P〈0.05）. On the 7th day, the TNF- α concentration in BALF in the combination treatment group was significantly lower than those in the model group, methylprednisolone treatment group and prostaglandin E1 treatment group （P〈0.05）; on the 14 th day, the TNF-α concentration in BALF in the combination treatment group was significantly lower than that in the model group （P〈0.05）; on the 28th day, the levels of TNF-α in the prostaglandin E1 treatment group and combination treatment group were significantly lower than that in the model group （P〈0.05）. Conclusion Prostaglandin E1 combined with Xuebijing injection may significantly inhibit TGF-β1expression in the lung tissue of rats with ac

关 键 词：血必净 前列腺素E1 转化生长因子-Β1 肿瘤坏死因子Α 急性肺纤维化 

分 类 号：R285.5[医药卫生—中药学]