吡格列酮对糖尿病大鼠肾脏TIMP-1、TGF-β1和PDGF-β表达的影响  

Effects of pioglitazone on the expression of TIMP-1, TGF-β1 and PDGF-βon kidney of diabetic rats

作　　者：熊盛池[1] 谢燕[1] 熊利 

机构地区：[1]四川省凉山彝族自治州第一人民医院内分泌科,四川西昌615000 [2]四川省喜德县两河口中心卫生院内科,四川喜德615000

出　　处：《中国医药科学》2012年第18期12-15,共4页China Medicine And Pharmacy

摘　　要：目的研究吡格列酮对糖尿病大鼠肾脏的保护作用及机制。方法建立链脲佐菌素诱导的糖尿病SD大鼠模型,将造模成功的糖尿病大鼠随机抽样分成以下4个组:吡格列酮组、模型组、厄贝沙坦组、吡格列酮组+厄贝沙坦组,并另设正常组以作对照。对每一组大鼠给予相应的措施干预处理12周。光镜观察肾组织病理结构改变;免疫组织化学法检测肾组织TIMP-1和TGF-β1表达;免疫蛋白印迹检测肾组织PDGF-β的表达。结果模型组肾组织病理结构改变明显,肾皮质TIMP-1、TGF-β1和PDGF-β表达显著升高;吡格列酮组、厄贝沙坦组和吡格列酮+厄贝沙坦组肾组织TIMP-1和TGF-β1和PDGF-β等指标显著低于模型组,差异均有统计学意义(P<0.05)。结论吡格列酮对DM大鼠的肾脏具有保护作用,保护机制与TIMP-1、TGF-β1和PDGF-β的在肾组织的表达减少有关。Objective To study the protective effects of the Pioglitazone on the kidney of diabetic rats. Methods The model of DM rats induced by STZ were randomly divided into the following four groups inclucling pioglitazone group, diabetic model group, group of irbesartan, pioglitazone group combined with irbesartan group and normal control group. For each group of rats were respectively intervened for 12 weeks by the corresponding measures, and renal histopathological change was observation by light microscopic. TIMP-1 and TGF-β1 in renal tissue were detected by immunohistochemical technique, and PDGF- β expression in renal tissue was detected by western blotting. Results The renal tissue histopathological of diabetic model group was markedly different from others group. The expression of TIMP-1,TGF-β1 and PDGF-β on renal cortex were increased in diabetic rats model group（P0.05）. Conclusion Pioglitazone has the protective effects in the kidney of diabetic rats. The protection mechanism of pioglitazone may be associated with reduction the expression of TIMP-1,TGF-β1 and PDGF-β.

关 键 词：吡格列酮 糖尿病肾病 基质金属蛋白酶组织抑制因子1 转化生长因子Β1 血小板衍化生长因子β 

分 类 号：R587.1[医药卫生—内分泌]