甲基阿魏酸对肝星状细胞增殖和细胞外基质的影响  

Influence of meth-ferulic acid on proliferation and extracellular matrix of rat hepatic satellite cells

作　　者：容明智[1] 李丽[1] 李勇文[1] 庞文肖[1] 程钰[1] 何善云[1] 冯小珍[1] 

机构地区：[1]桂林医学院,广西桂林541004

出　　处：《中国医药科学》2012年第23期32-34,共3页China Medicine And Pharmacy

基　　金：广西医科大学开放实验室项目(KFJJ2011-09)

摘　　要：目的研究甲基阿魏酸对大鼠肝星状细胞增殖和细胞外基质的影响。方法采用四甲基噻唑蓝法检测MFA对HSC的毒性;采用酶联免疫法法检测HSC上清液羟脯氨酸、金属蛋白酶组织抑制因子和透明质酸。结果 MFA可以抑制HSC的增殖,呈现剂量和时间依赖性;MFA各剂量组均可显著降低HSC细胞上清液HYP、HA、TIMP-1含量,呈现剂量依赖关系。结论 MFA通过抑制HSC的增殖,从而使ECM合成减少,肝纤维化减轻。Objective To study the influence of meth-ferulic acid on the proliferation and extracellular matrix（ECM） of hepatic satellite cells.Methods The toxicity to HSC caused by MFA was detected using the methyl thiazolyl tetrazolium method.The hydroxyproline,tissue inhibitor of metalloproteinase-1 and hyaluronic acid in the supernate of HSC were detected using the enzyme linked immunoabsorbent assay.Results MFA can inhibit the proliferation of HSC,showing dose and time dependence;all dosage groups of MFA can significantly reduce the HYP,HA,TIMP-1 contents in the supernate of HSC,showing dose and time dependence.Conclusion Through inhibiting the proliferation of HSC,MFA reduce the synthesis of ECM and relieve the hepatic fibrosis.

关 键 词：甲基阿魏酸 肝纤维化 肝星状细胞 细胞增殖 细胞外基质 

分 类 号：R512.62[医药卫生—内科学]