脂质体介导的反义寡核苷酸转染对体外培养人晶状体上皮细胞转分化的抑制效应  

Inhibition of transforming growth factor-β1-induced transdifferentiation by liposomal transfection of connective tissue growth factor antisense oligonucleotide in human lens epithelium cells

作　　者：庄华[1] 徐国兴[1] 

机构地区：[1]福建医科大学附属第一医院眼科,福州350000

出　　处：《临床眼科杂志》2012年第6期552-555,共4页Journal of Clinical Ophthalmology

基　　金：福建医科大学学科带头人培养基金课题(No.2007-XKDT)

摘　　要：目的通过脂质体介导转染,研究结缔组织生长因子(CTGF)反义寡核苷酸对体外培养人晶状体上皮细胞(HLECs)向纤维组织转分化的影响。方法评估脂质体介导转染对体外培养HLECs的转染率;通过脂质体转染CTGF反义寡核苷酸,测定第3代HLECs细胞生长曲线;采用RT-PCR检测细胞CTGF和α-平滑肌动蛋白(α-SMA)mRNA水平。结果脂质体转染法细胞导入反义核酸阳性率高,脂质体转染24 h后,转化生长因子(TGF-β1)能显著增加CTGF以及α-SMA mRNA的表达,CTGF反义寡核苷酸能抑制这种效应。结论脂质体介导的CTGF反义寡核苷酸转染能有效抑制HLECs TGF-β1诱导的CTGF与α-SMA mRNA表达上调。Objective To investigate the influence of liposomal transfection of CTGF-ASON on transdifferentiafian induced by transforming growth factor-β1 （ TGF-β1 ） in normal human lens epithelium cells （ HLECs ）. Methods CTGF- ASON was transduced into normal human lens epithelium cells by α-txansfection with Lipofectamine 2000. The tramfection ratio of FITC-labeled ASON was measured by fluoroscope and the proliferation of HLECs was measured by CCK-8 assay. The expression of CTGF and α-SMA was detected by RT-PCR. Result Lipesomal transfection was high. Culture in TGF- β1 caused profound increase of CTGF and α-SMA that can be inhibited by lipesomal transfection. Condusion Lipesomal transfection of CrGF-ASON can abrogate the influence of TGF-β1 on increase of CTGF and α-SMA mRNA in HLECs.

关 键 词：结缔组织生长因子 晶状体上皮细胞 反义寡核苷酸 脂质体 

分 类 号：R776.1[医药卫生—眼科]