PDTC对PV-杀白细胞素（PVL）诱导THP-1巨噬细胞NF-κB活化及炎性因子表达的影响  

Effect of PDTC on the NF-κB activation and the expression of inflammatory cytokines in TEIP-1 macrophages induced by rPVL

作　　者：周馨[1] 马筱玲[1] 常文娇[1] 张翠萍[1] 卜素[1] 谢强[1] 

机构地区：[1]安徽医科大学附属省立医院检验科,合肥230001

出　　处：《中华微生物学和免疫学杂志》2012年第10期850-854,共5页Chinese Journal of Microbiology and Immunology

摘　　要：目的探讨二硫代氨基甲酸吡咯烷（PDTC）对PV-杀白细胞素（panton-valentine leucoeidin，PVL）诱导THP-1巨噬细胞NF—κB及炎性因子表达的影响。方法实验分3组，PBS对照组、rPVL刺激组和PDTC处理组，在加入rPVL刺激前60min，给予100μmol／LPDTC预处理THP-1巨噬细胞。采用免疫组化检测NF-κB的移位，Western blot检测细胞胞质IKB蛋白和胞核NF-κB蛋白表达，ELISA法检测细胞上清IL-8和IL-6蛋白的表达，RT—PCR法检测IL-8和IL-6mRNA水平表达。结果PDTC处理组NF-κB阳性细胞明显减少，核蛋白表达降低，胞质IKB蛋白降解减少；IL-8和IL-6mRNA表达明显下调，蛋白分泌量分别由12．96ng／ml和6．55ng／ml降低到6．78ng／ml和3．88ng／ml，差异有统计学意义（P〈0．05）。结论PDTC可能通过抑制NF—κB的活性，从而降低IL-8和IL-6的表达，对PVL引起的炎性损伤具有一定的保护作用。Objective To investigate effect of PDTC on the NF-κB activation and the expression of inflammatory cytokines in THP-1 macrophages induced by rPVL. Methods The study was divided into three groups： PBS-treated control group, rPVL-treated group and PDTC group which was given 100 μmol/L PDTC at 60 rain before rPVL exposure. Immunohistochemistry method was used to test the translocation of NF-κB protein; the expression of NF-κB and IKB protein was analyzed by Western blot; RT-PCR and ELISA was performed to test expression of IL-8 and L-6 in THP-1 macrophages. Results Compared with rPVL- treated group, the activation of NF-κB and the expression of IL-8 and L-6 in PDTC group was significantly decreased. The protein secretions of IL-8 and IL-6 were reduced to 6.78 ng/ml, 3.88 ng/ml, receptively（ P 〈0.05 ）. Conclusion The inhibitor of NF-κB, PDTC, could significantly decrease the secretion of pro-inflammatory in THP-I macrophages by rPVL, and it suggested that PDTC played an important role in protecting tissues from damage induced by rPVL.

关 键 词：杀白细胞素 二硫代氨基甲酸吡咯烷 核因子κB IL-8 IL-6 

分 类 号：R515[医药卫生—内科学]