PDL2在人胎盘源间充质干细胞对外周血T细胞免疫调节中的作用  

The role of PDL2 in human placenta mesenchymal stem cell-mediated inhibition of peripheral blood T cells

作　　者：王国艳[1] 李广云[2] 林艳华 王斐斐[1] 张丽霞[1] 栾希英[1] 

机构地区：[1]滨州医学院免疫教研室,烟台264003 [2]山东省千佛山医院 [3]烟台市中心血站

出　　处：《中华微生物学和免疫学杂志》2012年第10期879-884,共6页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目（31270962）;山东省科技发展计划资助项目（201IGGH21818）;山东省医药卫生发展计划资助项目（2007HZ039）;滨州医学院科研启动基金资助项目（BY2007KYQD09）

摘　　要：目的研究程序性死亡配体2（programmed death ligand2，PDL2）在人胎盘源间充质干细胞（human placenta mesenchymal stem cell，hPMSCs）对外周血T细胞活化、增殖及周期免疫调节中的作用。方法RT-PCR、激光共聚焦（1aser scanning confocal microscopy，LSCM）及流式细胞术（flow cytometry，FCM）检测PDL2在hPMSCs上的表达；应用PDL2siRNA阻断PDL2在hPMSCs上的表达；密度梯度离心法分离纯化T细胞；FCM分析阻断PDL2后，hPMSCs对植物血凝素（PHA）刺激下T细胞活化及波佛脂（PMA）活化下T细胞增殖和周期的影响。结果hPMSCs高表达PDL2分子，PDL2siRNA能有效阻断PDL2在hPMSCs上的表达；FCM分析结果显示，hPMSCs能够抑制CD69在T细胞上的表达，但阻断PDL2后，CD69的表达与未阻断组相比无明显变化；hPMSCs能够显著抑制PMA活化的T细胞的增殖，且阻断PDL2后，其增殖指数被进一步上调；与未阻断组相比，处于G0／G1期的T细胞数量明显减少，处于S期的细胞数量明显增加。结论PDL2在hPMSCs上表达能够协同hPMSCs对外周血T细胞周期的抑制，进而抑制T细胞的增殖。Objective To investigate the effect of programmed death ligand 2 （PDL2） in human placenta derived mesenchymal stem cells（hPMSCs） mediated immunoregulafion on peripheral blood T cells activation, proliferation and cell cycle. Methods The expression of the PDL2 on hPMSCs was detected by RT-PCR, LSCM and FCM, respectively. Specific PDL2 siRNAs were transfected into hPMSCs via cath- odolyte liposome transfection method. T cells were sorted from healthy peripheral blood by gradient centrifu- gation. The expression of early activation phenotype, proliferation and cell cycle of T cells were analyzed by FCM. Results PDL2 siRNA could effectively block the expression of PDL2 which was highly expressed on hPMSCs. The expression of CD69 on T cells had no significantly difference in blocking groups compared with unblocking groups, hPMSCs could inhibit the proliferation of T cells induced by PMA, compared with that of unblocking groups, the number of the T cells in G0/Gl phase was decreased while the number of the T cells in S phase was increased in the blocking groups. Conclusion PDL2 expressed on hPMSCs could promote the inhibitory effect of hPMSCs on T cell cycle and proliferation.

关 键 词：间充质干细胞 胎盘 T细胞 PDL2 增殖 

分 类 号：R392[医药卫生—免疫学]