透明质酸/聚酰胺-胺三元纳米基因复合物的构建及其体外评价  

Construction and in vitro evaluation of hyaluronic acid/polyamidoamine ternary nano-gene polyplex

作　　者：王晓宇[1,2] 孙李平[2] 朱全刚[1] 张玮[1,3] 张敏[1] 张丽娟[1] 戴子渊[1] 高申[1] 

机构地区：[1]第二军医大学长海医院药学部,上海200433 [2]成都军区司令部直属工作部,成都610000 [3]解放军522医院药材科,洛阳471003

出　　处：《第二军医大学学报》2012年第12期1288-1292,共5页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(81172514;81101658)~~

摘　　要：目的在聚酰胺-胺(polyamidoamine,PAMAM)树状大分子与DNA形成复合物的基础上添加透明质酸(hy-aluronic acid,HA)形成三元纳米复合物,研究其基因递送性能的改变。方法配制不同电荷比的三元纳米复合物,测定其粒径和电位;选择MCF-7和MDA-MB-231细胞进行转染效率的测定,选择B16和MCF-7细胞进行细胞毒性的测定。结果和结论形成的三元纳米复合物大小均一,结构稳定。与单纯的PAMAM载体相比,增加HA提高了转染效率,降低了细胞毒性,适合用于基因的递送和转染。Objective To prepare the ternary nano-gene polyplex by adding hyaluronic acid （HA） into DNA/ polyamidoamine （PAMAM） binary nano-gene polyplex, and to probe into its gene transfection ability. Methods DNA and HA were mixed with PAMAM dendrimer in different charge ratios to form ternary nano-gene polyplex, and the size and zeta potential of the polyplex were measured. MCF-7 and MDA-MB-231 cells were used for transfection efficiency test; B16 and MCF-7 cells were used for cell viability assay. Results and conclusion The prepared ternary nano-gene polyplex has even size and stable structure. HA can enhance the gene transfection efficiency and reduce the cell toxicity compared with pure PAMAM, making it suitable for gene delivery and transfection.

关 键 词：聚酰胺-胺 透明质酸 三元纳米复合物 转染效率 

分 类 号：R915.2[医药卫生—微生物与生化药学]