大鼠无肛畸形形成的胚胎机制和α-SMA表达的研究  

The expression of α-SMA in rat embryos with anorectal malformation induced by retinoic acid

作　　者：郭俊斌[1,2] 李昭铸[1] 

机构地区：[1]哈尔滨医科大学附属第二医院,黑龙江哈尔滨150086 [2]南京医科大学第二附属医院

出　　处：《中国优生与遗传杂志》2012年第12期32-34,共3页Chinese Journal of Birth Health & Heredity

基　　金：黑龙江省教育厅基金资助项目(批准号:11531124)

摘　　要：目的研究维甲酸诱导形成大鼠肛门直肠畸形的胚胎发育过程和α-SMA表达模式,探讨肛门直肠畸形矫正术后便秘和便失禁产生的原因。方法利用ATRA诱导大鼠产生肛门直肠畸形胚胎,病理形态学方法观察对比妊娠12、13、14、16、18d时大鼠胚胎肠道系统发育过程,并且通过免疫组化染色进行α-SMA表达的检测,与对照组胚胎中α-SMA表达表达模式进行对比。结果实验组中80%胚胎具有肛门直肠畸形,对照组未见明显畸形。胚胎发育观察证实妊娠12～16d为肛门形成的关键时间,畸形组与对照组相比发育明显滞后而且机构异常,α-SMA表达强度随胎龄增加,与肠道系统分化程度同步,畸形组中表达区域分散且未见外括约肌部分表达分布。结论 ATRA诱导产生无肛畸形胚胎中肠道系统发育过程与α-SMA表达同步相关,畸形组括约肌部分发育异常和α-SMA表达异常可能是肛门直肠畸形矫正术后便秘和便失禁产生的原因。Objective： To explore the reasons of constipation and fecal incontinence following anoplasty operation through studying the relation of the pathogenesis of anorectal malformation（ARM） and the abnormal expressions of α-SMA induced by retinoic acid.Methods： The rat embryos with anorectal malformations were induced by all-trans retinoic acid（ATRA）.The expression pattern of α-SMA in the embryos were determined by immunohistochemistry on E12（the 12th gestational day）,E13,E14,E15,E16and E18.The data of the control group and the ARM group were compared and analyzed.Results： In the ATRA treated group 80% embryos have anorectal malformations and the control group embryos were normal.E12 to E16 was the most crucial time to the rat anorectal development.The expression level of α-SMA increased with gestational age and intestinal system differentiation degree.The expression area of α-SMA in the ATRA treated embryos was more disperse than the control group.Conclusions： The expression level of α-SMA was increased with intestinal system development.The abnormal expression pattern of α-SMA in the ATRA treated embryos maybe is the reason of constipation and fecal incontinence following anoplasty operation.

关 键 词：维甲酸 肛门直肠畸形 Α-SMA 

分 类 号：R726.5[医药卫生—儿科]