一种有效制备果树高分子量DNA的方法  

An Efficient Protocol for Preparation of High Molecular Weight DNA from Fruit Trees

作　　者：王倩[1] 曲雪萍[1] 张开春 贾建航[1] 石金峰[1] 金德敏[1] 王斌[1] 

机构地区：[1]中国科学院遗传研究所,北京100101

出　　处：《高技术通讯》2000年第4期1-3,共3页Chinese High Technology Letters

基　　金：北京市自然科学基金项目!(5982004)

摘　　要：与一般植物叶片相比 ,果树叶片因富含多酚和多糖类物质 ,制备高分子量DNA比较困难。我们建立了一个从果树叶片制备高分子量DNA的有效程序 ,按照该方法制备的五种果树HMWDNA ,无叶绿体和线粒体DNA污染 ,无大量的非特异小片段的混杂 。Compared with general plant leaves, fruit tree leaves are rich in polyphenols and polysaccharides. Therefore it is very difficult to prepare high molecular weight DNA from them. We developed an efficient high molecular weight (HMW) genomic DNA preparation procedure for fruit tree leaves. We successfully isolated HMW DNA from 5 species fruit tree leaves that represented the material background of fruit tree leaves. The HMW DNAs were with no chloroplast and mitochondrail DNA and little small molecular weight DNA contamination. It demonstrated that the HMW DNA was suitable for reproducible digestion by restriction enzymes, large genomic library construction and analysis of complex genome region.

关 键 词：高分子量DNA 果树 基因 遗传 

分 类 号：Q52[生物学—生物化学]