慢病毒介导EGFP转染骨髓间充质干细胞在胶质瘤模型中迁移分布的研究  

The Study of the Migration EGFP Lentivirus Transfection of Bone Mesenchymal Stem Cells in Glioma Model

作　　者：庞恒元[1] 李万超 靳旭亮[3] 郑永日[1] 王建交[1] 

机构地区：[1]哈尔滨医科大学附属第二医院神经外科,黑龙江哈尔滨150086 [2]七台河市中医医院,黑龙江七台河154603 [3]海林市人民医院,黑龙江海林157100

出　　处：《现代生物医学进展》2012年第31期6025-6028,共4页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科研基金项目(11541203);黑龙江省青年科学基金项目(QC08C95)

摘　　要：目的:如何证实骨髓间充质干细胞(BMSCs)是胶质瘤基因治疗中最好的药物载体?将增强型绿色荧光蛋白(EGFP)标记的大鼠骨髓间充质干细胞移植入大鼠C6胶质瘤模型脑内,观察骨髓间充质干细胞在肿瘤内的迁徙与定位。方法:贴壁法培养大鼠骨髓细胞获取纯化的BMSCs。慢病毒介导EGFP转染BMSCs,于荧光显微镜下观察EGFP的表达,并行流式细胞仪检测EGFP阳性转染率。利用立体定向仪将培养好的C6细胞注入大鼠脑内,建立大鼠脑内胶质瘤模型。将标记EGFP的BMSCs利用微量注射器注入模型鼠脑内;移植后第1,7天处死大鼠,用荧光显微镜观察BMSCs在肿瘤内的迁移分布。结果:实验成功建立了大鼠脑内胶质瘤模型。以EGFP标记的BMSCs在模型鼠脑内主动迁移分布于肿瘤内部及肿瘤与正常脑组织交界侧。结论:骨髓间充质干细胞可以作为肿瘤基因治疗的良好载体。Objective： How to verify that BMSCs are best drug carriers in glioma gene therapy？ by observing the migration and localization of BMSCs in tumor following transplantation of rat BMSCs labeled by EGFP in the brain of a rat model. Methods： Pure BMSCs were obtained by culturing rat bone marrow cell. EGFP expression was observed under fluorescent microscope and detected EGFPpositive transfection efficiency by flow eytometry after Lentivirus-mediated EGFP transfected BMSCs. Using stereotaxic apparatus, C6 cells were injected into rat brain to establish glioma models.BMSCs labeled by EGFP were injected into the rat brain using a microinjector. The rats were sacrificed at dl and d7 after transplantation. The localization of BMSCs in tumor were observed under a Fluorescence microscopy. Results： The glioma model in rat brain was established successfully. BMSCs labeled by EGFP can accumulate around tumor actively in rat brain. Conclusion： These suggested that BMSCs were a good carriers of gane therapy.

关 键 词：骨髓间充质干细胞 慢病毒载体 增强绿色荧光蛋白 C6细胞 基因治疗 

分 类 号：Q953[生物学—动物学] Q75