MDM2反义寡核苷酸对血管平滑肌细胞MDM2和p53表达的影响  

Effection of MDM2 Antisense Oligonucleotide on MDM2 and p53 in Vascular Smooth Muscle Cell

作　　者：尹雪松[1] 付鹏[2] 姜廷军[2] 赵长久[2] 彭城海[1] 任仲侨[1] 赵侃[1] 

机构地区：[1]哈尔滨医科大学附属第四医院急诊科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第四医院核医学科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2012年第31期6051-6054,共4页Progress in Modern Biomedicine

基　　金：黑龙江省自然科学基金项目(D2008-27);哈尔滨市科技创新人才研究专项资金项目(2011RFQYS100;2009 RFQXS038)

摘　　要：目的:研究小鼠双微体扩增基因(mouse double minute 2;MDM2)反义寡核苷酸(antisense oligonucleotide;ASON)对血管平滑肌细胞MDM2和p53表达的影响,探讨MDM2反义寡核苷酸包埋支架防治支架内再狭窄的可行性。方法:人工合成一段针对MDM2 mRNA的反义寡核苷酸,脂质体包裹不同浓度ASON转染兔血管平滑肌细胞,RT-PCR和Western-blotting检测MDM2反义寡核苷酸对兔血管平滑肌细胞MDM2和p53表达的影响。结果:不同浓度MDM2反义寡核苷酸作用于兔血管平滑肌细胞后,MDM2和p53 mRNA表达量各浓度组之间有显著性差异(P<0.01),MDM2和p53蛋白表达量各浓度组之间有显著性差异(P<0.01)。结论:MDM2反义寡核苷酸体外能够特异性抑制兔血管平滑肌细胞MDM2表达,提高细胞内p53基因表达量,MDM2反义寡核苷酸有望被进一步应用于药洗脱支架研究。Objective： To investigate the possibility of using MDM2 mRNA antisense oligonucleotide （ASON） on preventing restenosis following angioplasty, the effection of the MDM2 ASON on MDM2 and p53 was studied in vascular smooth muscle cells, Methods： An ASON targeted to MDM2 mRNA were synthesized. RT-PCR and Western-blotting were carried out to assay the MDM2 and p53 mRNA and protein level after vascular smooth muscle cells were transfected with liposome-coated ASON. Results： The mRNA level of MDM2 and p53 had significant different at different concertration ofASON （P〈0.01）. The protein level of MDM2 and p53 were significant different at different concertration of ASON （P〈0.01）. Conclusion： The MDM2 ASON can be specially hybridized to the MDM2 mRNA and inhibit MDM2 gene expression and increase p53 gene expression in vascular smooth muscle cell in vitro which provide a basis for it to be used in drug eluting stent study.

关 键 词：小鼠双微体扩增基因 平滑肌细胞 寡核苷酸 脂质体 

分 类 号：R817.5[医药卫生—影像医学与核医学]