黄芪对儿童哮喘T细胞亚群细胞周期的影响  

Effect of Astragalus on the Cell Cycle Distribution in T lymphocyte Subsets in Patients with Asthma Attack

作　　者：李丹丹[1] 孙立春[1] 陈雪松[1] 蔡莉[1] 

机构地区：[1]哈尔滨医科大学附属第三医院内四科,黑龙江哈尔滨150000

出　　处：《现代生物医学进展》2012年第31期6086-6088,共3页Progress in Modern Biomedicine

摘　　要：目的:观察黄芪对发作期哮喘患者T细胞亚群细胞周期的影响,揭示黄芪在哮喘治疗中的意义。方法:课题以儿童哮喘病人T细胞亚群为研究对象,对CD4+、CD8+T细胞进行分离,采用细胞培养技术,用黄芪对其进行体外处理,使用流式细胞仪测定经黄芪处理和未经黄芪处理的CD4+、CD8+T细胞的细胞周期分布情况。结果:儿童哮喘患者CD4+、CD8+T细胞分别在经黄芪处理和未经黄芪处理的条件下培养,在CD4+T细胞中,黄芪组的S期CD4+T细胞百分比显著低于对照组,有统计学意义(P<0.01);G1期细胞百分比显著高于对照组,有统计学意义(P<0.01)。在CD8+T细胞中,黄芪组的S期CD8+T细胞百分比低于对照组,有统计学差意义(P<0.05);G1期细胞百分比高于对照组,有统计学意义(P<0.05)。结论:黄芪抑制发作期哮喘患者CD4+、CD8+T细胞DNA合成,进而调节细胞周期使之停滞在G1期。黄芪对发作期儿童哮喘患者有一定的治疗意义。Objective： To investiagte the effect of astragalus on the cell cycle distribution in T lymphocyte subsets in patients with asthma attack. Methods： The CD4＋ CD8＋ T lymphocytes obtained from peripheral blood of 40 patients with asthma attack were incubated with astragalus and without astragalus were analyzed by flow cytometry on the cell cycle distribution. Results： CD4＋T lymphocytes with astragalus progressed into S phase were significantly lower than those without astragalus （P〈0.01）.But remained in G1 phase were significantly higher than those without astragalus （P〈 0.01）, CD4＋ T lymphocytes with astragalus progressed into S phase were lower than those without astragalus （P〈0.05）. But remained in G 1 phase were higher than those without astragalus （P〈 0.05）. Conclusions： Astragalus could inhibit CD4＋ CD8＋ T cell DNA synthesis, Adjust so as to adjust the cell cycle to stagnation in the G1 phase.

关 键 词：哮喘 黄芪 T细胞亚群 细胞周期 

分 类 号：R562.25[医药卫生—呼吸系统]