高体重指数支气管哮喘患者血清中IL-8、IL-10及INF-γ的研究及意义  

The Investigation of IL-8,IL-10 and INF-γ in Serum in the High Body Mass Index Asthma Patients

作　　者：焉春华[1] 赵帅[1] 于方飞[1] 于百全[1] 杨原荻[1] 张晓飞[1] 邵玉霞[1] 

机构地区：[1]哈尔滨医科大学附属第二医院呼吸科,黑龙江哈尔滨150086

出　　处：《现代生物医学进展》2012年第31期6092-6094,共3页Progress in Modern Biomedicine

基　　金：黑龙江省科技攻关项目(GC07C34602)

摘　　要：目的:探讨高体重指数支气管哮喘患者血清中IL-8、IL-10及INF-γ的变化及临床意义。方法:选择高体重指数支气管哮喘患者(36例)、正常体重指数支气管哮喘患者(32例)以及健康人(32例),采用双抗体夹心ELISA法检测其急性发作期和缓解期血清中IL-8、IL-10和INF-γ的水平。结果:①高体重指数支气管哮喘组与正常体重指数支气管哮喘组急性发作期血清中IL-8水平显著高于缓解期以及对照组的水平(P<0.05)。②在缓解期,高体重指数支气管哮喘组血清中IL-8水平仍高于正常体重指数支气管哮喘组和对照组的水平(P<0.05)。③在急性发作期,高体重指数支气管哮喘组和正常体重指数支气管哮喘组血清中IL-10水平均显著低于其在缓解期及对照组的水平(P<0.05)。④三组间血清INF-γ水平在急性期与缓解期均无明显差异(P>0.05)。结论:血清中IL-8是高体重指数支气管哮喘患者发病过程中的重要炎症因子,并且始终参与其中。IL-10可能是支气管哮喘的抑炎因子,其缺乏可能是导致哮喘患者急性发作的因素之一。Objective： To investigate the methods of vocal folds sections in a Canine model, arid provide reliable section methods for vocal fold experiment. Methods： 4 dogs were chosen, 2 underwent coronal paraffin sections, while 2 underwetlt hofizonfal paraffin sections. The general structure and the collagen of lamina propria （LP） were observed by HE staining and Masson trichrome staining, Results： In both coronal sections and horizontal sections the surface of vocal folds were covered with Stratified sqtlamous epithelium. There was a large number of tightly packed fibrous tissue mixed with a small amount of glands in the lamirla propria, and beneath the LP was muscle layer. The coronal situation of LP can be observed in coronal sections, and serial sections will be needed to detect the whole situation of vocal folds. The anterior parts, membranous parts and vocal process of vocal folds can be observed in horizontal sections with obvious anatomical landmarks. Masson trichrome staining shows thinner collagen fiber bundles can be observed in the superficial layer of the LP, in the Middle layer thicker and thinner fiber bundles are arranged together, and in the deep layer the thick fiber bundles are arranged closely.Conclusion： The coronal situation of LP can be observed in coronal sections, and serial sections will be needed to observe the whole situation of vocal folds. The anterior parts, membranous parts and vocal process of vocal folds can be observed in horizontal sections with obvious anatomical landmarks.

关 键 词：IL-8 IL-10 INF-Γ 支气管哮喘 高体重指数 

分 类 号：R562.25[医药卫生—呼吸系统]