高丹草超低氢氰酸含量ISSR特征片段的克隆及序列分析  

Cloning of ISSR Characteristic Fragments Related to Super Low Hydrocyanic Acid Content in SorghumSudangrass and Sequence Analysis

作　　者：周亚星[1,2] 于肖夏[3] 于卓[1] 李造哲[4] 

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019 [2]内蒙古民族大学农学院,内蒙古通辽028043 [3]中国农业大学农业部作物遗传改良重点实验室,北京100193 [4]内蒙古农业大学生态环境学院,内蒙古呼和浩特010019

出　　处：《中国草地学报》2012年第6期75-80,共6页Chinese Journal of Grassland

基　　金：国家自然科学基金项目(30960262);内蒙古农业综合开发办项目(2012-97)

摘　　要：为明确高丹草超低氢氰酸含量ISSR特征片段的DNA分子特征,以散穂高粱×黑壳苏丹草杂种F2的3个超低氢氰酸含量ISSR特征片段H-1、H-2、H-3和散穂高粱×白壳苏丹草杂种F2的3个超低氢氰酸含量ISSR特征片段B-1、B-2、B-3为材料,对6个不同的ISSR特征片段进行了克隆和测序分析。结果表明:克隆的特征片段H-1序列全长为1377bp,序列包含1个357bp的完整编码区(ORF),位于743～1099bp区域,共编码119个氨基酸,片段与小麦亚种蛋白b的同源性为60%。片段H-2序列全长2268bp,序列包含1个279bp的ORF,位于735～1013bp区域,共编码93个氨基酸,片段与高粱假设蛋白SORBIDRAFT_05g027190同源性为63.9%,与水稻粳稻组假设蛋白OsJ_07776同源性为62%。片段H-3序列全长2614bp,序列包含1个339bp的ORF,位于1024～1362bp区域,共编码113个氨基酸。片段B-1序列全长1432bp,序列包含1个300bp的ORF,位于700～999bp区域,共编码100个氨基酸。片段B-2序列全长1363bp,序列包含1个228bp的ORF,位于603～830bp区域,共编码76个氨基酸。片段B-3序列全长2751bp,序列包含1个186bp的ORF,位于2534～2719bp区域,共编码62个氨基酸。片段H-3、B-1、B-2、B-3与现有已知的植物碱基序列和氨基酸序列没有同源性,可能是与高丹草超低氢氰酸含量关系更为密切的DNA新序列。To make clear DNA molecular characteristics of ISSR characteristic fragments related to super low hydrocyanic acid content in Sorghum-Sudangrass,ISSR characteristic fragment H-1,H-2 and H-3 related to super low hydrocyanic acid content in hybrid F2 of loose spike Sorghum×black shell Sudangrass and ISSR characteristic fragment B-1,B-2 and B-3 related to super low hydrocyanic acid content in hybrid F2 of loose spike Sorghum×white shell Sudangrass were cloned and sequence was tested.The results showed that whole length of the cloned fragment H-1 was 1377bp,the sequence contained a complete coding region（ORF） of 1357bp in length,located in the 743bp～1099bp region,coding 119 amino acids,the homology of the fragment with that of wheat subspecies was 60%.Whole length of fragment H-2 was 2268bp,the sequence contained a complete coding region（ORF） of 279bp in length,located in the 735bp～1013bp region,coding 93 amino acids,the homology of the fragment with SORBIDRAFT_05g027190 of sorghum was 63.9%,and the homology of the fragment with OsJ_07776 of rice was 62%.Whole length of fragment H-3 was 2614bp,the sequence contained a complete coding region（ORF） of 339bp in length,located in the 1024bp～1362bp region,coding 113 amino acids.Whole length of fragment B-1 was 1432bp,the sequence contained a complete coding region（ORF） of 1300bp in length,located in the 700bp～999bp region,coding 100 amino acids.Whole length of fragment B-2 was 1363bp,the sequence contained a complete coding region（ORF） of 228bp in length,located in the 603bp～830bp region,coding 76 amino acids.Whole length of fragment B-3 was 2751bp,the sequence contained a complete coding region（ORF） of 1186bp in length,located in the 2534bp～2719bp region,coding 62 amino acids.Fragment H-3,B-1,B-2 and B-3 had no homology with known plant base sequence and amino acid sequence,they may be more closely related to super low hydrocyanic acid content of new DNA sequences.

关 键 词：高丹草 超低氢氰酸含量 ISSR特征DNA片段 克隆 测序 

分 类 号：S544[农业科学—作物学]