番茄抗枯萎病I-2基因的SNP分型  

SNP genotyping of Fusarium wilt resistance gene I-2 in tomato

作　　者：徐薪惟[1] 李景富[1] 姜景彬[1] 张贺[1] 康立功[1] 陈秀玲[1] 王傲雪[1] 许向阳[1] 

机构地区：[1]东北农业大学,哈尔滨150030

出　　处：《植物保护》2012年第6期22-26,共5页Plant Protection

基　　金：现代农业产业技术体系专项资金(CARS-25-A-15);国家科技支撑计划(2009BAD8B01);东北农业大学创新团队基金;东北农业大学博士启动基金(2009RC09);黑龙江省高等学校创新团队基金(2009td07);哈尔滨市科技创新人才研究专项资金(2011RFXXN031)

摘　　要：利用直接测序方法检测番茄抗感品种I-2基因的DNA序列,发现了2个SNPs。以其为3′端,设计等位基因特异引物及其互补引物,研究了特异引物3′端碱基错配类型和位置对等位基因特异PCR的影响,并对52份种质资源进行了SNP分型。结果表明在下游引物3′末端第1、2位引入错配碱基可以提高反应的准确性;引入C-T碱基错配的引物,退火温度在58℃时能把I-2基因的突变位点和感病品种的对应位点区分开来,为番茄抗枯萎病辅助育种提供了有力工具。Two SNPs were discovered by aligning the I-2 genes between resistant varieties and susceptible varie- ties. Allele-specific primers and their complementary primers were designed using the SNPs as their 3＇-end. In addition, we studied the effect of the mismatched bases at the 3＇-end of the allele-specific primers on PCR. To type these SNPs, 52 groups SNP genotypes were studied, with SNPs detected in tomato I-2 gene. The results showed that introducing proper mismatched bases at the first or the second sites of the 3＇-end of the antiprimer could improve the accuracy of the reaction, and the primer with C-T mismatch could distinguish mutation location of I-2 from its susceptible allele location at the annealing temperature 58 ℃. The results provided a powerful tool for Fusarium wilt resistance gene selection in tomato breeding program.

关 键 词：番茄 枯萎病 I-2基因 SNP 等位基因特异PCR(AS-PCR) 

分 类 号：S432.23[农业科学—植物病理学] S436.412[农业科学—农业昆虫与害虫防治]