柴黄益肾颗粒对糖尿病并发肝损伤大鼠TGF-β1及GLUT-1的调控作用研究  

Effect of Chaihuangyishen Granule on expression of glucose transporter-1 and transforming growth factorβ1 in the liver of diabetic nephropathy rats

作　　者：韩文兵[1,2] 李平[2] 赵婷婷[2] 张浩军[2] 路晓光[2] 古艳婷[2] 朱斌[2] 李忻[2] 杨鑫[2] 马会霞[3] 

机构地区：[1]北京中医药大学,北京100029 [2]中日友好医院临床医学研究所,北京100029 [3]河北联合大学,唐山063000

出　　处：《中日友好医院学报》2012年第6期348-352,F0004,共6页Journal of China-Japan Friendship Hospital

基　　金：国家自然科学基金(No.30973911;No.81173422);国家国际科技合作专项(No.2011DFA31860)

摘　　要：目的:观察柴黄益肾颗粒对糖尿病肾病并发肝损伤大鼠的肝保护作用,并探讨其可能机制。方法:单侧肾切除合并链脲佐菌素注射诱导糖尿病肾病大鼠模型,给药20周后,检测大鼠血糖、肝功能、肝组织病理、肝组织Ⅰ、Ⅲ型胶原及肝糖原蓄积程度,并检测肝组织葡萄糖转运蛋白-1(GLUT-1)、转化生长因子-β1(TGF-β1)mRNA及蛋白表达水平。结果:与假手术对照组比较,模型组大鼠肝组织表现为肝细胞索排列紊乱,炎性细胞浸润,糖原颗粒蓄积,Ⅰ、Ⅲ型胶原纤维异常增生,柴黄益肾颗粒组大鼠肝组织炎性细胞浸润、糖原蓄积程度及Ⅰ、Ⅲ型胶原纤维增生程度显著减轻,肝组织GLUT-1及TGF-β1 mRNA、TGF-β1蛋白表达显著低于模型组(P<0.05)。结论:柴黄益肾颗粒对糖尿病肾病并发肝损伤大鼠具有肝保护作用,这可能与柴黄益肾颗粒显著降低肝组织GLUT-1及TGF-β1表达有关。Objective：To investigate the effect of Chaihuangyishen granule （CHYS）the expressions of glucose transporter-1 （GLUT-1）and transforming growth factor β1 （TGF-β1）in the liver of diabetic nephropathy rats, and probe the mechanism.Methods：Male Wistar rats were randomly divided into 12 rats in sham control group and 100 rats in uninephrectomy groups which were induced into diabetic model by injection of 40mg/kg STZ.The diabetic rats were then randomly divided into model group,CHYS low dosage group,CHYS medial dosage group,CHYS high dosage group and Fosinopril-treated group.The body weight,plasma glucose were measured.The liver pathomorphology was observed by HE staining and PAS staining, and type I and III collagen were measured with immunohistochemical technique.Liver GLUT-1 and TGF-β1 expression were mea- sured with reverse transeriptase-polymerase chain reaction （RT-PCR） and Western blot.Results：The glycogen accumulation and fibration were alleviated in the liver tissues of rats treated by CHYS,accompanying the sup- pression of type I and m collagen expression in the liver.Liver expression of GLUT-1 and TGF-β1 de- creased significantly compared with the model group（P〈0.05）.Conclusions：CHYS have protective effect on ex- perimental liver injuries in diabetic nephropathy rats.The mechanism might be related to the suppression of expression of GLUT-1 and TGF-β1.

关 键 词：柴黄益肾颗粒 糖尿病肾病 肝损伤 葡萄糖转运蛋白-1 

分 类 号：R285.5[医药卫生—中药学]