重组葡激酶溶栓作用的实验研究  

Experimental researches on thrombolytic effects of recombinant staphylokinase

作　　者：陈飞虎[1] 杨军[2] 刘建辉 王静[2] 智强 刘颖雪 

机构地区：[1]安徽医科大学临床药理研究所,合肥230032 [2]安徽省医学科学研究所省生物医药重点实验室,合肥230061 [3]安徽省桑尼生物技术研究所,合肥230042 [4]安徽省阜阳市人民医院,阜阳236000

出　　处：《中国药理学通报》1999年第6期536-539,共4页Chinese Pharmacological Bulletin

摘　　要：目的　研究重组葡激酶对体内外血栓的溶解作用。方法　采用体外纤维蛋白溶解法，家兔体内纤维蛋白溶解试验，胶原蛋白肾上腺素诱发小鼠体内脑血栓形成模型及静脉结扎形成大鼠下腔静脉血栓模型。结果　重组葡激酶体外给药对纤维蛋白具有显著溶解作用，使家兔血浆优球蛋白溶解时间明显缩短，给药后０－５至２ｈ间血浆鱼精蛋白试验均呈阳性反应，但对血浆纤维蛋白原含量无显著影响；可显著降低脑血栓小鼠死亡率，缩短偏瘫动物的恢复时间；对结扎形成的大鼠下腔静脉血栓湿重有明显降低，给药后血栓所占血管面积明显减少。结论　重组葡激酶具有极显著溶解体内外血栓的作用，等剂量条件下，作用优于尿激酶。AIM To investigate thrombolytic effect of recombinant staphylokinase in vitro and in vivo . METHODS Model of cerebral vascular estublished by collagen adrenalin and model of inferior vena cava by ligation were used. RESULTS Recombinant staphylokinase possessed obvious effect on fibrin in vitro and lessened euglobulin lysis time. recombinant staphylokinase also reduced ratio of death of mice with cerebral thrombosis and decreased the weight of thrombus of inferior vena cava of rats remarkedly. CONCLUSION Recombinant staphylokinase possesses excellent thrombolytic effects on thrombus. At the same dosage its potency is superior to that of urokinase.

关 键 词：重组葡激酶 静脉血栓 脑血栓 溶栓疗法 

分 类 号：R973.2[医药卫生—药品] R743.320.5[医药卫生—药学]