N-甲基小檗胺对豚鼠单一心室肌细胞ATP敏感钾电流的影响  

Effect of N-methyl berbamine on ATP sensitive K + currents in single ventricular myocytes of guinea pig

作　　者：李金鸣[1] 张沈丽[1] 杨光[2] 肖庆桓 王玉萍[1] 张克义[1] 

机构地区：[1]中国医科大学药理学教研室,沈阳110001 [2]中国医科大学第一临床学院循环内科,沈阳110001

出　　处：《中国药理学通报》1999年第6期542-544,共3页Chinese Pharmacological Bulletin

摘　　要：目的　研究Ｎ甲基小檗胺对豚鼠单一心室肌细胞ＡＴＰ敏感钾电流的作用。方法　膜片钳制技术全细胞记录模式。结果　Ｎ甲基小檗胺抑制心室肌细胞ＡＴＰ敏感钾电流（ＩＫＡＴＰ）且具浓度依赖关系。指令电位为０ｍＶ时，３种浓度Ｎ甲基小檗胺（０－１，１，１０μｍｏｌ·Ｌ－１）可使ＩＫＡＴＰ分别由给药前的（０－４６±０－０９）ｎＡ，（０－４３±０－１５）ｎＡ和（０－４７±０－１０）ｎＡ减少至给药后的（０－３７±０－０７）ｎＡ（ｎ＝４，Ｐ＜０－０５），（０－２９±０－１８）ｎＡ（ｎ＝５，Ｐ＜０－０５）和（０－２１±０－０７）ｎＡ（ｎ＝４，Ｐ＜０－０５）。其抑制率分别为１８－８１％±６－１６％（Ｐ＜０－０５），４１－４７％±２４－０５％（Ｐ＜０－０５）和５５－００％±１２－９４％（Ｐ＜０－０５）。其它指令电位下的ＩＫＡＴＰ的改变也符合此趋势。结论　Ｎ甲基小檗胺阻断豚鼠心肌细胞ＡＴＰ敏感Ｋ＋钾通道。AIM To investigate the effect of N methyl berbamine(NMB)on ATP sensitive potassium currents( I KATP )in single ventricular myocytes of guinea pig. METHODS Patch clamp technique with whole cell configuration. Holding potential was -40 mV, commanding potential was -100～+50 mV and duration was 600 ms. Pipette solution contained 0 3 mmol·L -1 ATP. RESULTS NMB inhibited I KATP in a concentration dependent manner. When holding potential was -40 mV and command potential was 0 mV, I KATP were reduced from (0 46±0 09) nA, (0 43±0 15) nA, and(0 47±0 10) nA to (0 37±0 07) nA( n=4,P <0 05), (0 29±0 18) nA (n=5, P <0 05), and(0 21±0 07) nA( n=4, P <0 05) respectively after 0 1, 1, and 10 μmol·L -1 NMB were added to the external solution. The inhibitory rates were 18 81%±6 16%( P <0 05), 41 47%±24 05%( P< 0 05 ), and 55 00%±12 94% ( P <0 05) respectively. The changes of I KATP under other command potentials were consistent with this. CONCLUSION NMB inhibits ATP sensitive K + channel in single ventricular myocytes.

关 键 词：甲基小檗胶 心室肌细胞 ATP敏感钾电流 

分 类 号：R972[医药卫生—药品] R282.71[医药卫生—药学]