竹红菌素高产菌株的筛选与分子鉴定  

Screening and Molecular Identification of Hypocrellin-Producing Shiraia bambusicola

作　　者：夏更寿[1] 麻汉林[1] 

机构地区：[1]丽水学院,丽水323000

出　　处：《上海交通大学学报（农业科学版）》2012年第6期1-5,21,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：浙江丽水市科技计划项目(YL200735007;2010JYZB08);浙江省自然科学基金(LY12C14003)

摘　　要：研究利用薄层层析(TLC)以及高效液相色谱(HPLC)方法,对82株竹黄子实体真菌的液体发酵培养物进行了初筛和复筛,最终获得竹红菌素高产菌株ZHLS-03。经rDNA-ITS序列分析,该真菌与竹黄(Shiraia bambusicola,AY536374.1)相似度为100%。再结合其个体形态特征、菌落形态特征,确认该菌为竹黄无性型菌株。A hypocrellin-producing Shiraia bambusicola ZHLS-03 was isolated through the first screening with TLC and the second screening with HPLC from the liquid fermentation culture of 82 strains, rDNA- ITS sequence analysis indicated that its homology reached 100% alignment with Shiraia bambusicola （AY536374. 1） in Genbank. In addition,the result identified that ZHLS-03 was the anamorphosis strain of Shiraia barnbusicola when combined the analysis of its individual modality and colony modality character.

关 键 词：竹黄 竹红菌素 筛选 分子鉴定 

分 类 号：S567.39[农业科学—中草药栽培]