钩唇石斛离体快速繁殖技术体系的建立  

Establishment of Technological System for In Vitro Propagation of Dendrobium aduncum

作　　者：周音[1] 殷丽青[1] 汤桂钧 黄卫昌[3] 胡永红[3] 李秀芬[1] 蒋建平 刘炤[3] 胡海锋 

机构地区：[1]上海市农业科学院林木果树研究所,上海201403 [2]上海市闵行区农业科学研究所,上海201109 [3]上海辰山植物园,上海201602

出　　处：《上海交通大学学报（农业科学版）》2012年第6期62-65,71,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市科委资助项目(10dz1913100);上海市绿化管理局资助项目"兜兰和石斛等兰科植物种质资源收集;栽培和繁殖技术研究"

摘　　要：为了加快石斛类珍稀植物的育种进程、挽救珍稀濒危种类,为石斛类珍稀植物离体快速繁殖提供依据,本文以钩唇石斛(Dendrobium aduncum Lindl.)未成熟的种子为外植体进行离体快速繁殖研究。结果表明:未成熟种子接种于0.5～1.0mg/L MS+BA+0.2mg/L NAA培养基可以直接诱导原球茎;适宜原球茎增殖的培养基为2.0mg/L MS+BA+0.5mg/L KT+0.5mg/LNAA,增殖系数为12.4;原球茎分化的适宜培养基为0.5mg/L MS+BA+0.5mg/L KT+0.2mg/L NAA,分化率为97.5%;芽苗生根的适宜培养基为1/2MS培养基中添加0.5mg/L IBA+0.5mg/L NAA,平均根数为8.57条,生根率达100%,移栽后再生植株生长健壮,长势良好。In order to accelerate the breeding process, rescue rare and endangered variety and provide the premise for in vitro propagation of rare plant Dendrobium, in this study, the immature seeds used as explants were applied to conduct rapid in vitro breeding of Dendrobium aduncurn. The results showed that the protocorm was induced directly on MS medium containing 0. 5 - 1.0 mg/L of BA and 0. 2 mg/L of NAA. The optimal medium for proctocorm multiplication was 2.0 mg/L MS＋BA＋0. 5 mg/L KT＋0. 5 mg/L NAA with the propagation coefficient up to 12.4. The MS medium supplemented with 0. 5 mg/L BA,0. 5 mg/L KT and 0. 2 mg/L NAA was suitable for the differentiation of the adventitious buds with the differentiation percentage of 97. 5%. The optimal medium for root formation was MS 1/2＋0. 5 mg/L IBA＋0. 5 mg/L NAA. The number of root per plantlet was 8. 57 and the rooting rate up to 100%. The plantlets grew well after transplanted.

关 键 词：钩唇石斛 组织培养 快速繁殖 未成熟种子 

分 类 号：S682.31[农业科学—观赏园艺]