Erlotinib对鼻咽癌细胞株放射敏感性的作用  

Role of Erlotinib in radiosensitivity of nasopharyngeal carcinoma cell lines

作　　者：张秀萍[1] 张欢欢[1] 袁太泽[1] 梁颖[1] 黄赖机[1] 李健[1] 叶家才[1] 郑荣辉[1] 

机构地区：[1]广州医学院附属肿瘤医院放疗科,广东广州510095

出　　处：《暨南大学学报（自然科学与医学版）》2012年第6期579-582,590,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广州市科技局项目(2009Z1-E281)

摘　　要：目的:研究Erlotinib对人鼻咽癌细胞株CNE1及CNE2放射敏感性的影响。方法:人鼻咽癌细胞株CNE1、CNE2经Erlotinib、深部X线照射或两者联合处理,采用细胞克隆形成法检测Erlotinib对鼻咽癌细胞株放射敏感性的影响;流式细胞仪检测细胞凋亡及细胞周期的情况。结果:Erlotinib增强了鼻咽癌细胞株CNE1、CNE2的放射敏感性,放射增敏比分别为1.076、1.109;Erlotinib联合电离辐射可导致鼻咽癌细胞株CNE1、CNE2发生G2/M期阻滞;并促进鼻咽癌细胞株CNE2放射诱导的细胞凋亡。结论:Erlotinib联合电离辐射可致鼻咽癌细胞周期G2/M期阻滞,增强了鼻咽癌细胞株的放射敏感性。Aim：To explore the role of Erlotinib in radiosensitivity of human nasopharyngeal carcinoma CNE1 and CNE2 cell lines. Methods： Human nasopharyngeal carcinoma cells dealed with Erlotinib and/or Radiation.The effect of Erlotinib on radiosensitivity was detected by using clonogenic survival assay.Cell apototic rate and cell cycle were evaluated by flow cytometery. Results： Erlotinib enhanced radiation sensitivity of CNE1 and CNE2 cells,the SER was 1.076 and 1.109.Erlotinib combine with radiation induced G2 phase arrest of two cells,only induced the apoptosis of CNE2 cell. Conclusion：Erlotinib combine with radiation provoked G2 phase arrest enhanced radiation sensitivity.

关 键 词：鼻咽癌 电离辐射 表皮生长因子受体 厄洛替尼 

分 类 号：R319[医药卫生—基础医学]