IAA浓度对细基江蓠繁枝变种切段再生芽的诱导效果  

Effects of different concentrations of IAA on induction of regenerated buds from algal fragments in Gracilaria tenuistipitata var. liui Zhang et Xia

作　　者：钟晨辉[1] 黄瑞芳[1] 郑雅友[1] 李雷斌[1] 刘波[1] 林琪[1] 

机构地区：[1]福建省水产研究所,福建厦门361013

出　　处：《福建水产》2012年第6期481-487,共7页Journal of Fujian Fisheries

基　　金：海洋公益性行业科研专项(201105008);福建省属公益类科研院所基本科研专项(2011R1003-1);福建省水产研究所青年科学创新基金(014003)

摘　　要：研究了在MES液体培养基内添加0.1～10.0 mg·L-1IAA诱导细基江蓠繁枝变种藻体切段再生芽形成无性系的效果。结果显示,藻体切段只能在形态学上端产生再生芽是细基江蓠繁枝变种的特性。与对照组相比,培养液添加0.1～5.0 mg·L-1低浓度的IAA均能不同程度提高藻体切段切口处再生芽发芽率和生长率,其中添加2.0 mg·L-1IAA对藻段再生芽生长的促进效果最佳,培养至35 d时,再生芽平均长度达到(25.99±1.28)mm,明显高于对照组平均长度(18.50±0.92)mm。然而,培养液添加10.0 mg·L-1高浓度的IAA,反而抑制了藻段再生芽的形成与生长,相比于对照组,再生芽数量最少,发芽率和生长率最低,培养至35 d时,再生芽平均长度仅为(12.23±0.85)mm,低于对照组。研究结果表明,IAA诱导江蓠再生芽的最适宜浓度为2.0 mg·L-1,可为进一步构建细基江蓠繁枝变种种苗快繁应用技术提供基础。Effects of MES liquid medium with the concentrations of O. 1 - 10. 0 mg· L-1 IAA on induction ofregenerated buds from algal fragments in Gracilaria tenuistipitata var. liui Zhang et Xia were studied. The re- suits showed that the morphological upper ends of algal fragments characteristically germinated newborn buds that eventually developed into asexual lines. Compared to the control group, lower concentrations of 0. 1 -5. 0 mg· L-1 IAA added in liquid culture medium all improved the sprouting rates and growth rates of regenerated buds in various degrees, and the promotion effect of 2.0 mg· L-1 IAA was the best, and the mean length of regenerated buds was （25.99 ±1.28 ） mm, obviously higher than that of the control with （ 18.50± 0. 92 ） mm after 35 days of culture. However, the group with concentration more than 10. 0 mg· L-1 IAA restrained form- ing and growth of the regenerated buds, and made them with the lowest quantities, sprouting rates and growth rates, and the mean length of regenerated buds was only（ 12.23±0. 85）mm, lower than that of the control group after being cultured for 35 days. It was indicated that the optimum concentration of IAA for inducing re- generated buds of the species was 2.0 mg· L-1. These base conclusions would be benefit for establishing fur- ther applied technique of rapid propagation in G. tenuistipitata var. liu.

关 键 词：IAA 诱导 再生芽 细基江蓠繁枝变种 

分 类 号：S917.3[农业科学—水产科学]