HPLC测定槐花药材不同部位芦丁和槲皮素含量  

Content Determination of Rutin and Quercetin in Different Parts of Sophora Japonica L.Medicinal Materials by HPLC

作　　者：杜玉然[1] 贺福元[1,2,3] 周逸群[1] 谢相贵[1] 石继连[1,2] 

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]湖南中医药大学中药药性与药效国家中医药管理局重点实验室,湖南长沙410208 [3]湖南中医药大学现代中药制剂制备与评价实验室,湖南长沙410208

出　　处：《中国中医药信息杂志》2012年第12期43-45,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81173558);湖南省"十二五"重点学科中药学(1008);湖南省"十二五"重点学科药学(1007)

摘　　要：目的比较槐花不同部位(槐花、槐米及枝梗)中芦丁和槲皮素的含量。方法采用高效液相色谱法进行测定,Ultimate XB-C18色谱柱(4.6 mm×250 mm,0.45μm),水-甲醇为流动相梯度洗脱,流速1 mL/min,柱温35℃,检测波长256 nm,进样量20μL。结果芦丁含量为槐米>槐花>枝梗;槲皮素含量整体较低,且槐花>槐米>枝梗。结论槐花各部位芦丁含量较高,且含量差别不大。槐花中的槲皮素含量明显高于槐米及其枝梗。Objective To compare rutin and quercetin content of different parts （flower, bud and pedicel） of Sophora Japonica L.. Methods HPLC method was used with Ultimate XB-C18 column （4.6 mm×250 mm, 0.45 μm）, water and methanol as solvent system with gradient elution. The flow rate was 1 mL/min, column temperature was 35 ℃, detection wavelength was set at 256 nm, and injection volume was 20 μL. Results The content of rut_in was bud〉flower〉pedicel. The content of quercetin was low on the whole, and in sequence of flower〉bud〉pedicel. Conclusion In the different parts of Sophora Japonica L., rutin content is high with little difference. Quercetin content of flower is significantly higher than in bud and pedicel.

关 键 词：槐花 不同部位 芦丁 槲皮素 含量比较 

分 类 号：R284.1[医药卫生—中药学]