IL-18BP重组蛋白对TNF-α诱导的人脐静脉内皮细胞的作用研究  

The effect of IL-18 binding protein on the inflammation and apotosis of TNF-α-induced HUVECs

作　　者：李刚[1] 贺芙云[1] 常贺[1] 黄明山[1,2] 王焱[1,3] 

机构地区：[1]厦门大学附属中山医院厦门市心脏中心,361004 [2]福建医科大学协和临床医学院,福州350108 [3]厦门大学医学院,361005

出　　处：《免疫学杂志》2013年第1期19-23,共5页Immunological Journal

基　　金：国家自然基金资助项目(81270294);福建省自然基金项目(2012J01415);厦门市科技计划项目(3502Z20124054)

摘　　要：目的探讨人重组白细胞介素-18结合蛋白(rhIL-18BP)对肿瘤坏死因子(TNF-α)诱导的内皮细胞炎症、凋亡的影响。方法体外培养人脐静脉内皮细胞(HUVECs),用20 ng/ml的TNF-α和不同质量浓度的rhIL-18BP孵育相应时间,提取mRNA并用PCR及实时定量PCR检测细胞因子(IL-6、IL-8)、黏附分子(ICAM-1、VCAM-1)的mRNA表达;ELISA法检测细胞上清液中细胞因子(IL-6、IL-8)、可溶性黏附分子(sICAM-1、sVCAM-1)的蛋白含量;流式细胞术检测细胞凋亡的变化。结果 1)IL-18BP可以抑制TNF-α诱导的人脐静脉内皮细胞中炎症因子的表达;2)IL-18BP能抑制TNF-α诱导的HUVECs的凋亡。结论 rhIL-18BP对TNF-α诱导的内皮炎症反应有抑制作用,并可以抑制TNF-α诱导的内皮细胞凋亡。We aim to investigate the effects of interleukin-18 binding protein （IL-18BP） on the inflammation and apoptosis of human umbilical vein endothelial cell （HUVEC） induced by tumor necrosis factor-or （TNF-α）. HUVECs were treated with 20 ng/ml TNF-α and different concentrations of IL-18BP for corresponding times. The mRNA expressions of IL-6, IL-8, ICAM-1, and VACM were detected by PCR and Real-time PCR; the protein expressions of IL-6, IL-8, sICAM-1, and sVACM-1 were detected by ELISA. And the apoptosis was detected by flow cytometry detection. The results showed IL-18BP down-regulated the mRNA and protein expressions of IL-6, IL-8, ICAM-1, and VCAM-1. Furthermore, IL-18BP suppressed cell apoptosis of TNF-α-induced HUVECs. These results demonstrate that IL-18BP could inhibit the inflammatory response of endothelial cells to TNF-α, which suggested that IL-18BP exerts potential anti-inflammatory effect in modulating various inflammatory processes such as atherosclerosis.

关 键 词：白细胞介素-18结合蛋白 人脐静脉内皮细胞 炎症 凋亡 肿瘤坏死因子-Α 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]