类风湿关节炎患者外周血单个核细胞中PADI4 mRNA的表达及其意义  

The expression of PADI4 mRNA in peripheral blood mononuclear cells of patients with rheumatoid arthritis and its significance

作　　者：郭靖[1] 钱龙[1] 李向培[1] 厉小梅[1] 汪国生[1] 高登文[1] 

机构地区：[1]安徽医科大学省立医学风湿免疫科,合肥230001

出　　处：《免疫学杂志》2013年第1期43-46,63,共5页Immunological Journal

基　　金：2009年安徽省卫生厅医学科研课题(09A041)

摘　　要：目的检测类风湿关节炎(RA)患者外周血单个核细胞(PBMCs)肽酰基精氨酸脱亚氨酶4(PADI4)mRNA的表达,分析其与RA患者的临床指标的相关性,探讨PADI4在RA发病机制中的作用及意义。方法实时定量PCR检测RA组(60例)、正常对照组(40例)PBMCs中PADI4 mRNA表达,并分析其与抗环瓜氨酸肽(CCP)抗体、疾病活动指数DAS28评分、C反应蛋白(CRP)、红细胞沉降率(ESR)、类风湿因子(RF)及病程等指标的关系。结果 RA组PADI4 mRNA相对表达[34.6(16.7,70.8)],明显高于正常对照组[20.6(11.1,51.8)](P<0.05)。RA组中PADl4 mRNA表达与抗CCP抗体、DAS28评分水平、ESR、RF呈正相关(r=0.527,P<0.001;r=0.416,P=0.001;r=0.371,P=0.004;r=0.287,P=0.030),与CRP、病程无相关性(r=0.015,P=0.919;r=0.064,P=0.625)。结论 RA病人外周血PBMCs PADI4 mRNA表达显著增高,并与抗CCP抗体水平、DAS28评分、ESR、RF呈正相关,可能是RA病情活动的一个有用的指标,PADI4可能在RA的发病和病理过程中起重要作用。To detect the levels of PAD14 mRNA in peripheral blood mononuclear cells of rheumatoid arthritis （RA） patients and to explore its significance in the pathogenesis of RA, 60 RA patients and 40 healthy individual were recruited in the study. Real-time quantitative PCR was used to determine the expression of PAD14 mRNA was determined by real-time quantitative PCR. Furthermore, we analyzed the relationship between PAD14 mRNA level and clinical indexes in RA patients. We found that PAD14 mRNA level of RA was significantly higher than that of healthy controls （P〈 0.05）. And the PAD14 level was positively related to anti-CCP antibodies （r=0.527, P〈 0.001）, DAS28 score （r=0.416, P=0.001）, ESR （r=0.371, P=0.004）, RF （r=0.287, P=0.030）, but was not associated with CRP levels （r=0.015, P=0.919） or disease duration （r=0.064, P=0.625）. All above results suggested that PAD14 may play an important role in the pathogenesis of RA.

关 键 词：类风湿关节炎 抗环瓜氨酸肽抗体 肽酰基精氨酸脱亚氨酶-4 

分 类 号：R593.22[医药卫生—内科学]