MDCK细胞在流感病毒单克隆抗体鉴定中的应用  

Application of MDCK cells in characterization of influenza mAb

作　　者：张海祥[1,2] 齐宗利 刘扬[1,2] 谢鑫 郭华[1,2] 郭春艳 胡军[1,2] 

机构地区：[1]陕西省人民医院中心实验室 [2]西安交通大学医学院第三附属医院,西安710068 [3]西北大学生命科学学院,西安710069

出　　处：《免疫学杂志》2013年第1期64-66,92,共4页Immunological Journal

基　　金：国家科技重大专项"传染病监测技术平台"项目(2009ZX10004-202);陕西省重大科技创新项目(2007ZKC(一)06-02)

摘　　要：目的尝试应用MDCK细胞鉴定流感病毒单抗。方法以制备的流感病毒单克隆抗体做一抗,对感染流感病毒的MDCK细胞进行免疫荧光检测。结果与阴性对照细胞相比,感染病毒的MDCK细胞呈现出明显的绿色荧光。结论 MDCK细胞可以用于流感病毒单克隆抗体的鉴定,具有较好的适用性。The study aimed to characterize influenza virus monoclonal antibody （mAb） by Madin-Darby canine kidney （MDCK） cells. The monoclonal antibodies against influenza virus were prepared as the first antibody to detect the MDCK cells infected by H1N1 influenza virus with immunofluorescence assay （IFA）. The MDCK cells infected with H1N1 influenza virus exhibited green fluorescence compared with the negative control ceils. From the results we can conclude that MDCK cells could be used for characterization of the influenza virus monoclonal antibody and have better applicability.

关 键 词：MDCK细胞 流感病毒 单克隆抗体 鉴定 

分 类 号：R392.11[医药卫生—免疫学]