高速逆流色谱法(HSCCC)分离制备香水莲花中的柚皮素  

Separation and Preparation of Naringenin from Nymphaea hybrid by High-speed Countercurrent Chromatography

作　　者：姜洪芳[1,2] 石宝俊[2] 赵伯涛[2] 张卫明[1,2] 

机构地区：[1]南京师范大学,江苏南京210046 [2]南京野生植物综合利用研究院,江苏南京210042

出　　处：《中国野生植物资源》2012年第6期40-43,共4页Chinese Wild Plant Resources

基　　金：国家"十一五"支撑计划课题(2006BAD06B02);国家科技基础条件平台工作项目(2004DEA71010)

摘　　要：采用高速逆流色谱法对香水莲花中的黄酮类化合物进行了分离,溶剂系统为正己烷-醋酸乙酯-甲醇-水(体积比1∶1.2∶2∶1),上层为固定相,下层为流动相,流速2.0 mL/min,转速830 r/min,检测波长280 nm,进样量300 mg,分离出80 mg化合物C,经HPLC测定纯度>98.6%,根据UV光谱、MS分析及1H-NMR、13C-NMR图谱分析结果,化合物C鉴定为柚皮素,该化合物为首次从该植物中分离得到,为香水莲花的开发应用提供了理论依据。The flavonoid was successfully isolated and purified from Nymphaea hybrid by high-speed countercurrent chromatography（HSCCC） with a two-phase solvent system composed of n-hexane-ethylacetate-methanol-water（ 1： 1.2： 2： 1,v/v/v/v）. The lower phase was used as the mobile phase and the upper phase was used as the stationary phase. The flow rate was 2.0 mL/min, the revolution speed was 830 r/min and the detection wavelength was set at 280 nm. Under the optimized conditions, within 1.5 h, 80 mg of compound I with the purity of 98.6% by high performance liquid chromatography（HPLC） was obtained from 300 mg of the elution flow of silicagel column. The structure was confirmed as naringenin by using mass spectrometry,^ 1 H-nuclear magnetic resonance （^1H-NMR） and ^13 C-NMR . This compound was obtained from the plant for the first time.

关 键 词：高速逆流色谱法 高效液相色谱法 香水莲花 柚皮素 

分 类 号：R284.2[医药卫生—中药学]