上调HCCR-2基因表达对乳腺癌细胞增殖与凋亡的影响  

Effect on the Proliferation And Apoptosis in Human Breast Cancer Cells by Transfection of Gene HCCR-2

作　　者：桂春[1] 贾卫光 林梅[3] 戴桂红[4] 朱晓蔚[4] 肖蔚[4] 焦霞[4] 于鸿[4] 

机构地区：[1]江苏省泰州市人民医院普外科,江苏泰州225000 [2]江苏省泰州市人民医院心胸外科,江苏泰州225000 [3]江苏省泰州市人民医院肿瘤科肿瘤科,江苏泰州225000 [4]江苏省泰州市人民医院病理科,江苏泰州225000

出　　处：《临床医学工程》2012年第12期2117-2119,共3页Clinical Medicine & Engineering

基　　金：泰州市人民医院重大科研资助项目(201008);江苏省六大人才基金(2011-WS-023)

摘　　要：目的利用细胞转基因技术探讨人宫颈癌癌基因-2(HCCR-2)对人乳腺癌细胞增殖、凋亡的影响及其可能的分子机制。方法经脂质体介导将含有HCCR-2真核表达质粒转染人乳腺癌MCF-7细胞株,采用G418筛选阳性细胞克隆及Western blot鉴定;Western blot检测阳性细胞克隆Bcl-2、Bax蛋白表达改变;流式细胞仪检测细胞凋亡率;噻唑盐(MTT)比色法检测阳性细胞克隆的增殖活性。结果成功建立高表达HCCR-2的阳性MCF-7细胞克隆(M-23),并证实其Bcl-2蛋白表达与细胞增殖活性均显著增高,而Bax蛋白表达与细胞凋亡显著降低。结论上调MCF-7细胞HCCR-2基因后,细胞增殖活性增加,细胞凋亡降低,其作用机制与Bcl-2表达增加而Bax表达降低。Objective To evaluate the effect and the possible mechanism of human cervical cancer oncogene-2 （HCCR-2） transfec tion on cell proliferation and apoptosis of human breast cancer cells. Methods pcDNA3.0- HCCR-2 and pcDNA3.0 were transfected into MCF-7 cells through lipofectamine and positive clones were screened by G418. Western blot were employed to identify protein expression of HCCR-2 in MCF-7 cells. The protein expressions of Bcl-2 and Bax in positive clones were detected by Western blot. The cell proliferation and apoptosis were observed by MTT assay and flow cytomertry. Results Positive clone M-23 with HCCR-2 over-expression was success fully established. Compared with other two groups, the expression of Bcl-2 and the proliferation of M-23 were significantly increased; how ever, the expression of Bax and the apoptosis of M-23 were significantly decreased. Conclusions HCCR-2 transfection can effectively pro mote the proliferation and inhibit the apoptosis of MCF-7 cells by upregulating the expression of Bcl-2 and downregulating the expression of Bax in MCF-7 cells.

关 键 词：人宫颈癌癌基因 BCL-2 BAX 凋亡 

分 类 号：R318[医药卫生—生物医学工程] R737.9[医药卫生—基础医学]