抗日本血吸虫膜蛋白单克隆抗体NP11-4轻链可变区基因的分离克隆及测序研究  

CLONING AND SEQUENCING OF THE V_L GENE OF A McAB AGAINST THE MEMBRANE PROTEIN OF SCHISTOSOMA JAPONICUM

作　　者：俞小淙[1] 蒋欣[1] 尹长城[1] 张众[1] 林晴[1] 管晓虹[2] 黄华梁[1] 

机构地区：[1]中国科学院遗传研究所,北京100101 [2]南京医科大学寄生虫学教研室

出　　处：《中国人兽共患病杂志》2000年第3期22-24,共3页Chinese Journal of Zoonoses

摘　　要：本文报告了从抗日本血吸虫单抗NP11- 4杂交瘤细胞株的基因组DNA中 ,用PCR技术扩增出抗体轻链可变区基因 ,并克隆至pUC19质粒中进行测序分析。研究表明 :该基因长 336bp ,编码 112个氨基酸 ,经结构分析及与抗体库中一般序列的比较 ,证实该序列为轻链可变区基因 ,属κ轻链SubgroupII亚类 ,由种系基因中的V与Jκ 2基因重排而成。The V L gene of a McAb against the membrane protein of Schistosoma japonicum was amplified from the genomic DNA of the hybridoma NP11-4 by PCR The PCR products were cloned into pUC19 Vector Several recombinants that had been inserted with the target fragment were sequenced by Sangers method The result showed that the V L was 336bp long, encoding 112 amino acids It belongs to the mouse Vκ subgroup II and rearrangs from V gene and Jκ 2 gene

关 键 词：轻链可变区基因 PCR 日本血吸虫 单克隆抗体 

分 类 号：R532.21[医药卫生—内科学]