酯酶同工酶鉴定细粒棘球蚴细胞系(13G-5)种属来源  

IDENTIFICATION OF CELL LINE OF ECHINOCOCCUS GRANULOSUS BY ANALYSIS SOD ISOENZYME PATTERN

作　　者：陆家海[1] 许键[2] 郭中敏[3] 李德昌[4] 郭固[3] 

机构地区：[1]兰州军区乌鲁木齐总医院,乌鲁木齐830000 [2]新疆农业大学 [3]新疆畜牧科学院 [4]解放军农牧大学

出　　处：《中国人兽共患病杂志》2000年第3期56-57,共2页Chinese Journal of Zoonoses

基　　金：军队医药卫生青年基金!资助课题 96Q0 2 3

摘　　要：目的　鉴定细粒棘球蚴细胞系细胞的种属来源。方法　应用SDS凝胶电泳测定细粒棘球蚴细胞系酯酶同工酶 (EST) ,并和E granulosus原头节、囊液及包虫病人血清作比较。结果　细粒棘球蚴细胞系、E granulosus原头节、囊液含有相同的酯酶同工酶谱 ,在同一位点出现相同的一条酶带。结论　实验结果表明从包虫病人培育的 13G - 5细胞确系来源于E granulosus细胞。Aim Identifying the original cell line of Echinococcus granulosus Methods SOD isoenzyme patters of cells from E granulosus cell line,E granulosus protoscoleces and cyst fluid and hydatidosis patient had been analysed through polycrylamid gel electrophoresis Result The SOD isoenzyme band of E granulosus cell line were same as that of the protoscolex and cyst fluid,but more markedly from that of from patient Conclusion It indicates that the cell line of E granulosus(13G-5)in which was from hydatid patient and protoscoles cells are homogeneous This study had laid foundation for identifying the cell line of E granulosus from its origin

关 键 词：细粒棘球蚴细胞系 酯酶同工酶 种属来源 鉴定 

分 类 号：R383.33[医药卫生—医学寄生虫学]