染毒方式对球孢白僵菌侵染茶卷叶蛾毒力的影响  

Effect of Inoculation Method on Toxicity of Beauveria bassiana against 5^(th) Instar Larvae of Homona coffearia

作　　者：王定锋[1] 曾明森[1] 李慧玲[1] 王庆森[1] 吴光远[1] 

机构地区：[1]福建省农业科学院茶叶研究所,福建福安355015

出　　处：《福建农业学报》2012年第10期1097-1101,共5页Fujian Journal of Agricultural Sciences

基　　金：国家茶叶产业技术体系项目(CARS-23);国家国际科技合作项目(2010DFB33030-3);福建省现代农业(茶叶)产业技术体系建设项目;福建省财政专项--福建省农业科学院科技创新团队建设项目(CXTD2011-23)

摘　　要：探讨球孢白僵菌JYBb201和JYBb202菌株在4种染毒方式下对茶卷叶蛾5龄幼虫的毒力,结果表明,当孢子悬液浓度为1.0×107个.mL-1时,球孢白僵菌JYBb201和JYBb202菌株对茶卷叶蛾5龄幼虫的最终校正死亡率分别为:浸虫法85.00%和81.94%;涂抹法82.50%和76.94%;浸梢法47.50%和43.61%;喷雾法52.50%和48.61%。同时,2个菌株采用浸虫法处理茶卷叶蛾5龄幼虫僵虫率也最高,分别为67.50%和57.50%;致死速度最快,LT50分别为3.510d和3.808d。Toxicities of Beauveria bassiana strains,JYBb201 and JYBb202,against the 5th instar larvae of Homona coffearia were compared when 4 different methods of inoculation were applied.The adjusted mortality rates of H.coffearia larvae subjected to 1.0×107 conidia·mL-1 spore suspensions of JYBb201 and JYBb202 were found to be 85.00% and 81.94%,respectively,when the larvae were submerged in the spore suspension;82.50% and 76.94%,respectively,when the larvae were smeared with the spore suspension;47.50% and 43.61%,respectively,when the tea tips were dipped in the suspension;and,52.50% and 48.61%,respectively,when the spore suspension was sprayed on the larvae.The immersing method also appeared to bring about the highest cadaver rates at 67.50% for JYBb201 and 57.50% for JYBb202.It was the same when the LT50 values were used for the comparison 3.510 d for JYBb201 and 3.808 d for JYBb202.

关 键 词：球孢白僵菌 茶卷叶蛾 染毒方式 毒力 

分 类 号：Q965[生物学—昆虫学]