血小板源性生长因子促进大鼠血管平滑肌细胞CD44和连环蛋白表达的研究  

Effect of platelet-derived growth factor on the expressions of CD44 and β-catenin in vascular smooth muscle cell of rats

作　　者：于悦卿[1] 王超[2] 刘凌[3] 耿瑞丽[1] 王蓓[4] 谢霞[1] 白明晨[1] 郝玉宾[2] 

机构地区：[1]河北省人民医院检验科,石家庄市050051 [2]河北省人民医院临床医学研究中心,河北省老年医学重点实验室 [3]河北医科大学第一医院心内科 [4]河北省人民医院妇产科

出　　处：《河北医药》2012年第24期3685-3688,共4页Hebei Medical Journal

基　　金：河北省卫生厅资助项目(编号:03022)

摘　　要：目的研究血小板源性生长因子作用下,大鼠血管平滑肌细胞表达CD44和连环蛋白的变化,探讨两者对血管平滑肌细胞增殖和迁移的影响。方法体外原代培养大鼠血管平滑肌细胞,用MTT法检测经血小板源性生长因子处理后不同时间点对平滑肌细胞的增殖情况;用细胞迁移实验检测平滑肌细胞以血小板源性生长因子处理后的迁移情况;血小板源性生长因子刺激血管平滑肌细胞0min、20min和6h后,用基因芯片和RT-PCR技术检测血管平滑肌细胞表达CD44和连环蛋白的变化。结果 MTT法检测结果 :血小板源性生长因子培养血管平滑肌细胞6h、12h及24h后,细胞增殖活力分别是对照组的1.44、2.14和3.05倍(P<0.05);细胞迁移实验:血小板源性生长因子作用于细胞20min和6h后,细胞迁移活性分别是对照组的1.58倍和3.37倍(P<0.05);基因芯片筛查和RT-PCR确认:血小板源性生长因子培养血管平滑肌细胞20min和6h后,CD44基因表达分别是对照组(0min)的3.1倍和5.0倍(P<0.05);血小板源性生长因子对血管平滑肌细胞表达连环蛋白有明显诱导作用,作用20min和6h后的基因表达是对照组(0min)的3.77倍和5.46倍(P<0.05)。结论血小板源性生长因子可在较短的时间内显著提高血管平滑肌细胞CD44和连环蛋白的表达,同时明显促进平滑肌细胞的增殖和迁移。Objective To investigate the effects of platelet-derived growth factor （PDGF）on the expressions of CD44 and β-eatenin in vascular smooth muscle cell of rats, and to explore the influence of CIM4 and β-catenin on cell proliferation and migration of vascular smooth muscle ceils （VSMCs）. Methods The primary culture VSMCs of rats were treated by PDGF, then the cell proliferation of VSMCs at different time points was detected by MTT. The cell migration condition of VSMCs treated by PDGF was detected by cell migration test, and the changes of expression of CD44 and β-catenin of VSMCs at 0min, 20min,6h after stimulated by PDGF were detected by gene chips and RT-PCR. Results The proliferation activity of VSMCs treated by PDGF at 6h, 12h, 24h was as 1.44,2. 14,3.05 times as that in control group （ 0min ）, respectively, which suggested that PDGF obviously promoted the proliferation of VSMCs （ P 〈 0. 05 ）. The examination results of cell migration test showed that the cell migration activity of VSMCs 20rain ,6h after treated by PDGF was as 1.58 and 3.37 times as that in control group,which suggested that the cell migration activity of VSMCs was significantly increased. The results of gene chips screening and RT-PCR confirmed further that the expression of CD44 of VSMCs 20min,6h after treated by PDGF was as 3. I and 5.0 times as that in control group（ P 〈 0.05） ,which suggested that PDGF had obvious induction effect on the expression of β-catenin of VSMCs, and the gene expression 20min, 6h after exposure to PDGF was as 3.77 and 5.46 times as that in control group （ P 〈 0.05 ）. Conclusion PDGF can enhance the expression of CD44 and β-catenin of VSMCs in a shorter time. meanwhile, PDGF can significantly promote the proliferation and migration of VSMCs.

关 键 词：血小板源性生长因子 血管平滑肌细胞 CD44 连环蛋白 增殖 迁移 凋亡 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]