毛竹萌发种子全长cDNA文库的构建  

Construction of Full-length cDNA Library for Germinating Seeds of Phyllostachys heterocycla

作　　者：胡陶[1] 姚娜[2] 杨学文[3] 李雪平[1] 牟少华[1] 马艳军[1] 高志民[1] 彭镇华[1,2] 李潞滨[2] 

机构地区：[1]国际竹藤中心国家林业局竹藤科学与技术重点开放实验室,北京100102 [2]中国林业科学研究院林业研究所,北京100091 [3]赤峰市农牧科学研究院,内蒙古赤峰024031

出　　处：《安徽农业科学》2012年第35期17007-17010,17028,共5页Journal of Anhui Agricultural Sciences

基　　金：国际竹藤中心基本科研业务费专项(10618-10);国际竹藤中心基本科研业务费专项(12618-7);林业公益性行业科研专项(200704001)

摘　　要：[目的]构建毛竹萌发种子的全长cDNA文库。[方法]以毛竹萌发种子为材料,利用Oligo-capping法构建其全长cDNA文库。[结果]试验得到文库的库容达650万克隆,空载较少;插入片段大小在0.3～5.0 kb之间,片段大小的分级严格且一致性良好,其中显著的是长片段全长cDNA(1.0～5.0 kb)的比例高达30%,达到了高质量全长cDNA文库的标准。[结论]毛竹萌发种子全长cDNA文库的成功构建将为竹类植物功能基因组研究奠定重要的前期科研基础。[Objective] This study aimed to construct the full-length cDNA library for germinating seeds of Phyllostachys heterocycla.[Method] Germinating seeds of P.heterocycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method.[Result] The constructed library has a capacity of 6.5×106 recombinant clones in total,and a low proportion of clones without inserted fragmenst;the size of inserted fragments ranges between 0.3-5.0 kb,with strict classification and ideal consistency.Furthermore,the proportion of clones with long inserted fragments（1.0-5.0 kb） was as high as 30%,achieving the standard for high-quality full-length cDNA library.[Conclusion] The full-length cDNA library for germinating seeds of P.heterocycla was successfully constructed,which laid important foundation for the functional genomics research of bamboo plants.

关 键 词：毛竹 全长CDNA文库 萌发种子 Oligo-capping法 

分 类 号：S795.8[农业科学—林木遗传育种]