一种用于转基因水稻快速检测的DNA提取方法  

A Rapid DNA Extraction Method for Detection of Transgenic Rice

作　　者：苟小清[1,2] 付振艳[1] 王晓军[1] 

机构地区：[1]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011 [2]中国科学院研究生院,北京100049

出　　处：《安徽农业科学》2012年第35期17014-17015,17018,共3页Journal of Anhui Agricultural Sciences

基　　金：中国科学院"西部博士"资助项目(XBBS200914);新疆维吾尔自治区自然基金会项目(2011211B507)

摘　　要：[目的]建立一种快速筛选转基因水稻的DNA提取方法。[方法]对试剂盒法和快速法2种DNA提取方法进行比较,并分别用转Ta NADP-ME1和Ta NADP-ME2基因的转基因水稻进行验证。[结果]用快速法提取得到的DNA进行PCR扩增,可分别得到约1 700 bp的Ta NADP-ME2基因和约1 900 bp的Ta NADP-ME1基因,且条带清晰明亮,与试剂盒法相比无显著差异。[结论]该研究中快速提取DNA的方法经济、简便、快捷,适合用于对大量转基因样品进行检测。[Objective] To establish a rapid DNA extraction method for the screening of transgenic rice.[Method] The kit method and fast method of two DNA extraction methods were compared,and were proved by Ta NADP-ME1 and Ta NADP-ME2 gene transgenic rice respectively.[Result] PCR was done by using the DNA which extracted by the rapid method as template,about 1 700 bp Ta NADP-ME2 gene and about 1 900 bp Ta NADP-ME1 gene were obtained respectively,and the strips were clear and bright,comparing with the kit method showed no significant difference.[Conclusion] The rapid DNA extraction method of this study is economic,simple and quick,suitable for the large transgenic samples detection.

关 键 词：转基因水稻 DNA快速提取 PCR模板 

分 类 号：S511[农业科学—作物学]