石榴皮多酚对人乳腺癌细胞MDA-MB-231增殖及凋亡的影响  

Effects of extract of pomegranate peel on proliferation and apoptosis of breast cancer cell line MDA-MB-231

作　　者：章迅[1,2] 吴艾平[3,4] 章永红[3,4] 

机构地区：[1]江苏省中医药研究院 [2]江苏省中西医结合医院,210028 [3]南京中医药大学第一临床医学院 [4]江苏省中医院,210029

出　　处：《天津中医药大学学报》2012年第4期214-217,共4页Journal of Tianjin University of Traditional Chinese Medicine

基　　金：江苏省自然科学基金重点项目(BK2002201)

摘　　要：[目的]研究石榴皮多酚对人乳腺癌细胞MDA-MB-231增殖及凋亡的影响。[方法]四甲基偶氮唑盐(MTT)法检测石榴皮多酚对人乳腺癌MDA-MB-231细胞增殖抑制作用;流式细胞仪分析细胞周期及细胞凋亡。[结果]石榴皮多酚对人乳腺癌细胞MDA-MB-231的增殖有抑制作用,且呈现浓度和时间依赖性;石榴皮多酚能诱导MDA-MB-231细胞凋亡,使癌细胞周期被阻滞在G2-M期。[结论]石榴皮多酚可抑制人乳腺癌细胞MDA-MB-231增殖,诱导癌细胞凋亡,并阻滞癌细胞在G2-M期。[Objective] To investigate the effects of extract of pomegranate peel on proliferation and apoptosis of breast cancer cell line MDA-MB-231. [Methods] MTT assay was used to measure the anti-proliferation effect of extract of pomegranate peel in breast cancer line MDA-MB-231. The apoptosis and the cell cycle were analyzed by flow cytometry. [Results] The extract could inhibit the proliferation of breast cancer cell line MDA-MB-231 in dose- and time-dependent manner. It could induce the apoptosis of MDA-MB-231 cells. The growth of cancer cells was arrested in G2-M phase. [Conclusion] Extract of pomegranate peel can inhibit the proliferation of breast cancer cell line MDA-MB-231, induce apoptosis and block the growth of cancer cells in G2-M phase.

关 键 词：石榴皮多酚 乳腺癌 凋亡 MDA—MB-231细胞 

分 类 号：R285.5[医药卫生—中药学]