Sequence and organization of the mitochondrial genome of an urostylidid bug,Urochela quadrinotata Reuter(Hemiptera:Urostylididae)  

红足壮异蝽Urochela quadrinotata Reuter线粒体基因组的测定与分析(英文)

作　　者：戴宇婷[1] 李虎[1] 姜培[1] 宋凡[1] 叶子[1] 袁向群[2] 戴勋 常剑 彩万志[1] 

机构地区：[1]中国农业大学昆虫学系,北京100193 [2]西北农林科技大学昆虫博物馆,陕西杨凌712100 [3]红塔集团技术中心,云南玉溪653100

出　　处：《Entomotaxonomia》2012年第4期613-623,共11页昆虫分类学报（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(30825006,31061160186,31111140015);the Special Fund for Agroscientific Research in the Public Interest(201103012,201103022);the Natural Science Foundation of Beijing (6112013)

摘　　要：The complete mitochondrial genome of Urochela quadrinotata Reuter was sequenced and analyzed. This represents the first sequence completed for an insect in the family Urostylididae. The genome is 16585 bp in size and contains 35 genes （including 13 protein-coding genes, 20 transfer RNA genes, two ribosomal RNAs and a control region） with an A＋T content of 75.4%. Two tRNA genes, tRNATle and tRNAGln, are not detected in this genome. The gene order is similar to that most commonly found in insects. All tRNAs possess the typical clover-leaf structure, except tRNASer（AGN）, in which the dihydrouridine （DHU） arm forms a simple loop. The secondary structure of rrnL consists of six structural domains and 43 helices, and the rrnS includes three structural domains and 27 helices. An obvious feature of the control region is the presence of 1652 bp long tandem repeat sequences comprising 16 tandem repeat units.研究测定并分析了红足壮异蝽Urochela quadrinotata Reuter的线粒体基因组全序列。该线粒体基因组全长16585bp(GenBank登录号为JQ743678),A+T含量为75.4%,共编码35个基因,包括13个蛋白质基因、20个tRNA基因(两个tRNA基因,即tRNAIle和tRNAGln未被检测到)、2个rRNA基因及一段较长的非编码区(控制区,亦称A+T富含区)。基因排序与大部分昆虫的线粒体基因排列方式相同,没有发生基因重排。除tRNASer(AGN)的DHU臂无法形成典型的茎环结构,其余tRNA基因均能稳定形成典型的三叶草二级结构。预测了红足壮异蝽16S和12S rRNA的二级结果,分别包括6个结构域43个茎环和3个结构域27茎环。控制区含一个长1652bp的串联重复区域,由16个串联重复单元组成。

关 键 词：Urostylidid  mt genomics RNA second structure 

分 类 号：Q969[生物学—昆虫学]