狼尾珍珠菜下胚轴无性系建立研究  

Establishment of the Clone from Hypocotyl of Lysimachia barystachya

作　　者：赵溦[1] 富天思[1] 郭鑫英[1] 杨文新[1] 姜长阳[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116081

出　　处：《特产研究》2012年第4期38-41,共4页Special Wild Economic Animal and Plant Research

基　　金：辽宁省普通高等教育本科教学改革研究项目(201203041-4);辽宁省大学生创新创业训练项目(201203015011)

摘　　要：为保护野生资源和实现栽培,以狼尾珍珠菜下胚轴为材料,采用组织培养的方法,进行了愈伤组织诱导与分化、不定芽生根、试管苗生根继代增殖、移栽和定植的研究,建立起下胚轴的无性系。结果表明:MS+ZT 0.2mg/L+2,4-D 2.4mg/L是愈伤组织诱导培养的理想培养基;MS+AgNO30.5mg/L+KT1.5mg/L+NAA 0.2mg/L是愈伤组织分化培养的理想培养基;用浓度为10mg/L IAA溶液对不定芽处理24h,在White+ABT2号2.0mg/L的培养基培养的方法是不定芽生根培养的理想培养方法;White+ABT2号2.0mg/L是狼尾珍珠菜试管苗生根继代增殖培养的理想培养方法。试管苗移栽成活率为94.2%;定植成活率为98.3%。定植的试管苗保持了野生狼尾珍珠菜的植物学性状。The aim was to preserve the wild resources and meet the needs of cultivation, using tissue culture method, hypocotyl of Lysimachia barystachya were used as material to do the research on callus inducement and differentiation, rooting of adventitious buds, rooting multiplication of tube seedlings, transplantation of tube seedlings and stable planting, then the clone from hypocotyl was established. The results indicated that the best medium for callus induction was MS＋ ZT 0.2mg/L ＋ 2,4 - D 2.4mg/L;MS ＋ AgNO3 0.5mg/L＋ KT 1 .Smg/L＋ NAA 0.2mg/L was suitable for callus differentiation; cultivated in the medium White ＋ NO. ABT 2 2.0mg/L after the adventitious buds disposed 24h with IAA 10mg/L was the best method for rooting of adventitious buds;White ＋ NO. ABT 2 2.0mg/L was the best medium for rooting multiplication of tube seedlings; the transplanting survival rate of tube seedlings was 94.2 % and stable planting survival rate was 98.3 %, the tube seedlings sur- vived and retained all botany characteristics of the wild Lysimachia barystachya.

关 键 词：狼尾珍珠菜 愈伤组织 无性系 

分 类 号：S567.239.043[农业科学—中草药栽培]