高效液相色谱法与化学发光酶免疫分析法测定丙戊酸血药浓度的比较  

Comparison of HPLC and CLEIA for Determining the Serum Valproic Acid Concentrations

作　　者：范毅[1] 钱南萍 陈斌[1] 

机构地区：[1]江苏省泰州市人民医院,江苏泰州225300

出　　处：《中国药业》2012年第23期8-9,共2页China Pharmaceuticals

摘　　要：目的比较高效液相色谱法与化学发光酶免疫分析法测定丙戊酸血药浓度的结果,为临床合理用药提供依据。方法用两种方法分别测定46例使用丙戊酸的患者用药前的血药浓度,比较两种方法测定值的相关性。结果两种测定方法的线性关系回归方程为Y=0.980 2 X+2.477 7(r=0.979 3),两种方法测定结果差异无显著性(P>0.05)。结论高效液相色谱法与化学发光酶免疫分析法测定丙戊酸血药浓度具有相关性,不需相互校正,均可用于丙戊酸血药浓度的常规监测。Objective To compare the results from the determination of the serum valproic acid concentrations by HPLC and CLEIA. Methods Using HPLC and CLEIA to determine the serum concentrations of valproic acid was used in treatment of 46 cases before using medicine,then compared the relativity of the two methods. Results Linear regression examination is used for the two methods ,the linear regression function was Y = 0. 980 2X ＋ 2. 477 7（ r = 0. 979 3）. There were no significant statistical differences between the results determined by two methods（ P 〉 0. 05）. Conclusion There is good relativity between the serum valproic acid concentrations by HPLC and CLEIA. The determination results didn＇t need mutual correction. Both methods can be used for routine determination of the serum valproic acid concentrations.

关 键 词：丙戊酸 高效液相色谱法 化学发光酶免疫法 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R927.2[医药卫生—药学]