香蕉ACS启动子酵母单杂交报告载体的构建  

Report Vectors Construction and Effect Analysis of Banana ACS Promoter′s Yeast One-hybrid Method

作　　者：张建斌[1] 张建平[1] 贾彩红[1] 刘菊华[1] 金志强[1,2] 徐碧玉[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101 [2]中国热带农业科学院海口实验站,海南海口570101

出　　处：《热带农业科学》2012年第11期26-30,共5页Chinese Journal of Tropical Agriculture

基　　金："十二五"农村领域国家科技计划(No.2011AA10020605);海南省自然科学基金项目(No.809038)

摘　　要：对香蕉ACS基因启动子进行分析,选取关键元件通过人工合成和套叠PCR技术得到一段191 bp长度含有3个重复元件的DNA序列,把该序列构建报告载体pMmakeHIS2,经过酵母单杂交实验,结果表明:载体构建成功,单杂交筛选转录因子效果良好。pMmakeHIS2转化效率达到1.6×106个克隆/3μg,酵母单杂交获得156个阳性克隆,实验为研究香蕉ACS基因调控因子打下基础。The ACS gene promoter of banana was analyzed to select key components, while 191 bp DNA sequence containing three repetitive elements was obtained by synthetic and nested PCR techniques, and then constructed reporter vector pMmakeHIS2. The result show that vector build was successful, screening transcription factor effect was good. The conversion efficiency of pMmakeHIS2 was 1.6 × 106 clones / 3μg, 156 positive clones was screened using yeast one-hybrid. The experiment laid a foundation for the study of ACS gene regulatory factors in banana.

关 键 词：香蕉 ACS启动子 载体构建 酵母单杂交 

分 类 号：S668.1[农业科学—果树学]