禹白附凝集素的凝集活性及加热对刺激性作用的影响  

Hemagglutination Activity of Agglutinin from Typhonium giganteum Engl. and its Stimulation Effects by Heated

作　　者：刘先琼[1,2] 吴皓[1] 郁红礼[1] 潘耀宗[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]湖北中医药大学药学院,湖北武汉430061

出　　处：《南京中医药大学学报》2012年第6期548-551,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81173549;30973939);江苏省普通高校研究生科研创新计划项目(CXZZ11_0786)

摘　　要：目的研究禹白附凝集素(Typhonium giganteumagglutinin,TGA)的凝集活性以及加热对家兔眼刺激性作用的影响。方法考察不同来源红细胞、温度、pH及糖对TGA凝集活性的影响;通过Western Blotting方法分析禹白附毒针晶与TGA;采用家兔眼刺激性试验,考察加热禹白附毒针晶及TGA对家兔眼刺激性作用的影响。结果 TGA能凝集兔、鼠、狗的红细胞,不能凝集人及鸡的红细胞,在50℃内凝集活性稳定,pH 6.0～9.0范围内凝集活性最高,TGA凝集活性不被D-木糖、D-核糖、D-阿拉伯糖、无水葡萄糖、D-半乳糖、D-甘露糖、L-鼠李糖、甲基?α?D-甘露糖苷所抑制,仅被去唾液酸胎球蛋白所抑制;禹白附毒针晶与TGA均能与兔抗禹白附血清结合,在相同的位置显示相同的条带;家兔眼刺激试验结果显示单用TGA无刺激性,禹白附毒针晶中加入TGA刺激性显著增强,而禹白附毒针晶加上加热处理的TGA,其刺激性毒性与单独使用禹白附毒针晶的作用无差异,禹白附毒针晶加热处理后与禹白附毒针晶比较刺激性明显降低。结论 TGA具有较好的凝集活性,禹白附毒针晶中含有TGA,加热可破坏禹白附凝集素的刺激性作用。OBJECTIVE To study the hemagglutination activity of agglutinin from Typhonium giganteum（TGA） and its stimulation effects by heated.METHODS Studying on hemagglutination activity of TGA was used by different concentrations of rabbit,rat,dog,chicken and human red blood cells,temperature,pH and sugars.Raphides of Typhonium giganteum and TGA were contrastive analysis by western blotting.The stimulation effect of TGA by heated was observed in model of conjunctival congestion of rabbit.RESULTS TGA agglutinate rabbit,rat and dog red blood cells（RBCs） but is inactive towards human and chicken RBCs.The haemagglutinating activity of TGA was stable up to 50 ℃ for 20 min without any loss of activity and is not markedly affected by pH in the range of 6.0～9.0.The haemagglutination activity of TGA was inhibited by asialofetuin only,while monosaccharide is not react.The western blotting analysis showed that raphides of Typhonium giganteum and TGA were a single strap at the same site.The heated TGA was not aggravated the conjunctival congestion of rabbit by raphides of Typhonium giganteum.CONCLUSION TGA was hemagglutination activity.Raphides of Typhonium giganteum was comprised of TGA.The stimulation effects of raphides of Typhonium giganteum and TGA were decreased by heated.

关 键 词：禹白附凝集素 凝集活性 刺激性作用 

分 类 号：R285[医药卫生—中药学]