HPLC测定肾药Ⅲ号结肠定位片中芦丁、大黄素和大黄甲素的含量  

Study on Quality Standard of Nephropathy Medication Ⅲ Colon-specific Tablets

作　　者：邹浪[1] 王哲[2] 郭晓秋[1] 王勇[2] 王亚琴[2] 杨佳柳[2] 

机构地区：[1]江西中医学院附属医院,江西南昌330006 [2]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《南京中医药大学学报》2012年第6期577-579,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江西省卫生厅中医药科研计划(2010A036)

摘　　要：目的建立肾药Ⅲ号结肠定位片的质量标准。方法采用HPLC法测定了肾药Ⅲ号中芦丁、大黄素和大黄素甲醚的含量。结果芦丁、大黄素和大黄素甲醚在一定浓度范围内线性关系良好,芦丁、大黄素和大黄素甲醚线性范围分别为2.7～54.0μg/mL(r2=0.999 9)、1.1～22.0μg/mL(r2=0.999 3)和1.17～21.94μg/mL(r2=0.999 2),平均加样回收率分别为99.04%(RSD=2.04%,n=6)、101.21%(RSD=1.01%,n=6)和94.7(RSD=1.5%,n=6)。结论此方法简单准确,重现性良好,能有效控制肾药Ⅲ号结肠定位片的质量。OBJECTIVE To establish quality standard for Nephropathy medication Ⅲ colon-specific tablets.METHODS HPLC methods were established for the determination of Rutin,Emodin and Physcione.RESULTS The results of HLPC showed good linear relationships.The detection concentration ranges of Rutin,Emodin and Physcione were 2.7～54.0μg/mL（r2=0.999 9）,1.1～22.0 μg/mL（r2=0.999 3） and 1.17～21.94 μg/mL（r2=0.999 2）.The average recovery rate were 99.04%（RSD=2.04%,n=6）,101.21%（RSD=1.01%,n=6） and 94.7（RSD=1.5%,n=6）.CONCLUSION The method is simple,accurate and reproducible;it could be applicable in quality control of Nephropathy medication Ⅲcolon-specific tablets.

关 键 词：HPLC 薄层色谱 芦丁 大黄素 大黄素甲醚 

分 类 号：R284.1[医药卫生—中药学]