振荡频率对变形链球菌生长及黏附的影响  

Effects of Shaking Frequency in Growth and Adhesion behaviour of Streptococcus Mutans

作　　者：魏焱[1] 吕锦[1] 孔敏[1] 王斌[1] 刘劲松[2] 

机构地区：[1]成都市第一人民医院,四川成都610041 [2]温州医学院附属口腔医院,浙江温州325027

出　　处：《现代临床医学》2012年第6期456-458,共3页Journal of Modern Clinical Medicine

摘　　要：目的:探讨不同频率振荡培养对变形链球菌生长及其在修复材料表面黏附的影响。方法:将热凝树脂和钴铬合金试件分别置于含变形链球菌悬液的试管内,在不同振荡频率培养24小时。测定细菌黏附量及培养液细菌量。结果:当热凝树脂组振荡频率>60 r/min,钴铬合金组振荡频率>30 r/min时,变形链球菌黏附量随振荡频率的增加而减少(P<0.05)。振荡频率<90 r/min时,培养液中细菌随振荡频率增加而增加(P<0.05);振荡频率>90 r/min时,菌量随振荡频率增加而减少(P<0.05)。结论:当达到一定频率时,振荡频率与变形链球菌生长及黏附量呈负相关关系,提示口腔功能活动有利于减少细菌的生长繁殖以及修复体表面的早期黏附,有利于减少戴用义齿后口腔继发疾患。Objective：To study the influence of streptococcus mutans （S. mutans） adhesion to dental restorative materials and the growth of S. mutansin shaking culture with different frequencies. Methods：The specimen of acrylic resin and Co - Cr alloy were put into culture medium with S. mutans and incubated respectively in shaking condition with different frequencies for 24h. The amount of S. mutans on the surface of specimen and in the culture media was determined with the clone forming unit （ CFU ） counting method. Results ： When the frequency exceeded 60 r/min in acrylic resin groups and 30 r/min in Co - Cr alloy groups, the adhesive microbial amount declined with the increasing of shaking frequency （ P 〈 0.05 ） . When the frequency was between 0 -90 r/min, the amount of S. mutans in the culture media ascended with the increasing of shaking frequency （ P 〈 0.05 ） , but decreased when the frequency increased beyond 90 r/min （P 〈 0.05）. Conclusion：When the frequency exceed a certain level, the adhesion of S. mutans on the surface of two dental materials and the number of bacteria in the culture negatively correlate with the frequency, which implies that oral functional movements may lead to reduce microbes on the surface of dental restorative materials.

关 键 词：变形链球菌 热凝树脂 钴铬合金 振荡 培养 

分 类 号：R378.12[医药卫生—病原生物学]