^(13)C尿素呼气试验质谱仪的重复性及影响因素  

Study on repeatability detection and influence factors of ^(13)C-urea breath test with mass spectrometer

作　　者：王渊恺[1] 刘淼[1] 陆云[1] 张光明[1] 朱汇庆[1] 李鼎[1] 刘兴党[1] 

机构地区：[1]复旦大学附属华山医院核医学科,上海200040

出　　处：《中国医学装备》2012年第12期40-42,共3页China Medical Equipment

摘　　要：目的:13C质谱法尿素呼气试验是对幽门螺旋杆菌检测的"金标准",检验该方法的重复性并找出其影响因素。方法:使用双通道进样气体核素比值质谱仪,以13CO2超量δ值为结果,对无感染幽门螺旋杆菌的受检者所吹的气样,做Friedman按秩的双向方差分析。感染幽门螺旋杆菌的受检者在服用13C标记的尿素后所吹的气样,18根试管在放置2～10h后重复测量4次,做配对t检验。结果:阴性试管测试:复测所得δ值(±SD)为-24.54±0.86,-24.37±0.81,平均秩分别为1.16,1.84;P<0.001,复测δ值升高。5组δ值平均秩分别为1.77,1.77,2.98,3.63,4.86;P<0.001,5次测量结果逐渐升高。阳性试管测试:18对δ值平均秩分别为1.28,1.89,2.89,4.06,4.89,P<0.001。以上检验结果差异均有统计学意义。结论:13C质谱法测量幽门螺旋杆菌尿素呼气试验,其试管不能过多复测。长时间放置的试管或机器温度的变化会对δ值有影响,但变化较小。Objective：The ＂gold standard＂ of H.pylori detection is 13 C-urea breath test with mass-spectrometer.Reproducibility of mass spectrometry will be investigated.Methods：It was Dual-channel sample gas-isotope ratio mass spectrometry to obtain and analyze the results——the 13 CO2 excess values of δ.The gas sample in vitro is the first blowing breath of people who is not infected with H.pylori before taking the 13 C labeled urea.Then the Friedman two-way analysis of variance according to rank of the data was carried out analysis of repeatability.The second blowing breath were gas sample detected of people who had been infected with H.pylori with the mass spectrometry assay confirmed after taking the 13 C labeled urea.Then 18 tubes which had been placed 2-10h were detected by ＂replicates 3＂ and analyzed by paired t-test.Results：Negative in vitro test：δ values the twice retest obtained（±SD） was-24.54±0.86,-24.37±0.81,paired t-test t=-7.657,P0.001.Friedman rank two-way ANOVA analysis showed that the average rank of the two groups were respectively 1.16,1.84,P0.001.This two statistical methods showed that the retest results were elevated and statistically significant.According to Friedman rank twoway ANOVA analysis,the mean rank of δ values were respectively 1.77,1.77,2.98,3.63,4.86,P0.001,there was statistically significant.Positive test-tube test：according to the rank of two-way analysis of variance results,the average rank of 18 pairs of the δ values were,respectively,1.28,1.89,2.89,4.06,4.89,P0.001.This two statistical methods proved that the measurement results rose with the increasing frequency（time variation）,and there was a significant difference.Conclusion：13 C mass spectrometric analysis of H.pylori urea breath test.Results will be affected by the test tubes long-time placed,changes in the temperature of the machine,but the influence is not obvious.In the mass spectrometric analysis of the urea breath test and relevant experiments,each test tube should be detected as soon as

关 键 词：呼气试验 质谱法 碳同位素 可重复性 结果 幽门螺旋杆菌 

分 类 号：TH776.2[机械工程—仪器科学与技术]