秦川牛瘤胃微生物DNA提取方法的比较试验  

Comparison Test of DNA Extraction Methods from Rumen Microbe of Qinchuan Cattle

作　　者：徐文华 韩金涛[2,3] 蔡涛 辛小玲 白延琴 刘超 辛亚平[3] 

机构地区：[1]榆阳区牛家梁镇畜牧兽医工作站,陕西榆林719000 [2]甘肃省舟曲县江盘乡政府,甘肃舟曲746300 [3]西北农林科技大学动物科技学院,陕西杨凌712100 [4]陕西省渭南市畜牧技术推广中心,陕西渭南714300 [5]陕西富平县畜牧兽医工作站,陕西富平711700 [6]陕西子长畜牧兽医局史家畔畜牧兽医站,陕西子长717300 [7]陕西省洛川县畜牧局,陕西洛川727400

出　　处：《中国牛业科学》2012年第6期41-44,49,共5页China Cattle Science

基　　金：国家863项目(2010AA10Z101;2011AA100307;2011ZX08007-002;2011KTCL02-11;2012BAD12B07);MATS-Beef Cat-tle System;陕西省农业攻关项目(2010K02-13)资助

摘　　要：[目的]本试验的目的是优化秦川牛瘤胃微生物DNA提取方法。[方法]针对秦川牛特有的生理及生物学特性,采取珠磨法、反复冻融法、液氮研磨法、超声波法提取微生物DNA,并获得一种提取秦川牛瘤胃胃微生物DNA的最佳方法。[结果]珠磨法、反复冻融法提取的DNA片段较完整,DNA含量较高。[结论]珠磨法和反复冻融法是较科学的提取方法。[Objective]The purpose was to optimize DNA extraction method from rumen microorgan isms in Qinquan cattle. [Method]The bead mill, repeated freezing and thawing method, liquid nitrogen, grinding, ultrasonic were taken to extract microbial DNA according the unique physiological and biological characteristics of Qinchuan cattle. [Result]The result showed that the integrity and content of DNA fragments were better by bead mill, repeated freezing and thawing methods. [Conclusion]The results showed that the selected bead mill and repeated freeze thaw methods were better method to extract DNA from rumen microorganisms in Qinquan cattle.

关 键 词：秦川牛 瘤胃微生物 DNA 提取方法 

分 类 号：S823.2[农业科学—畜牧学]