棉花栽培品种/品系的SSR标记遗传多样性分析  

SSR Analysis of the Genetic Diversity of Cotton Cultivars/Lines

作　　者：滕中华[1] 王晓雯[1] 张建[1] 刘大军[1] 张正圣[1] 

机构地区：[1]西南大学农学与生物科技学院/南方山地农业教育部工程研究中心,重庆400716

出　　处：《西南大学学报（自然科学版）》2012年第12期20-26,共7页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(31071464;30900910)

摘　　要：选取4个棉花栽培种的29份棉花品种/品系,利用分布在棉花26条染色体的67对SSR多态性引物进行标记基因型检测,共检测出341个等位基因变异,每对引物的等位位点数在2～9之间,平均为5.1个.29份棉花品种/品系的遗传相似系数在0.53～0.97之间,平均值为0.73.遗传相似系数为0.78时,可将29份材料分为陆地棉、海岛棉和二倍体栽培种3个大组.遗传相似系数为0.84时,可将22份陆地棉品种/品系分成7亚组,同时将二倍体栽培种的中棉和草棉各分为一组.本研究表明,棉花栽培种陆地棉、海岛棉、中棉和草棉种内遗传差异小,种间杂交渐渗系的培育是增加陆地棉遗传多样性的有效途径.Twenty-nine cotton accessions from 4 cultivated species （Gossypium hirsutum, G. barbadense, G. arboretum and G. herbaceum） were selected as the experiment materials and their genotypes were detected with 67 SSR polymorphic markers anchored on the 26 cotton chromosomes. A total of 341 allelic variations were obtained, and each primer pair produced 2 to 9 loci, or 5.1 loci/primer on the average. The genetic similarity coefficient of the 29 accessions ranged from 0.53 to 0.97, with an average of 0.73. When coefficient was at 0.78, all the accessions were clustered into 3 groups, G. hirsutum, G. barbadense, and G. arboreum-G, herbaceum. When coefficient was at 0.84, the 22 G. hirsutum cultivars/lines were clustered into 7 sub-groups, and G. arboretum and G. herbaceum were clustered into two different groups. The above results showed that the intra-specific genetic diversity is low in cultivated cotton species, and introgression breeding is an effective approach to increase the genetic diversity of G. hirsutum.

关 键 词：棉花 SSR 遗传多样性 

分 类 号：S562[农业科学—作物学]